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Mass spectrometry-based pipeline for screening hazardous proteins in alternative protein-based foods

ABSTRACT: We present an effective non-targeted workflow for screening hazardous proteins in alternative protein-based foods (APBFs) that includes a protocol for efficient sample preparation, an optimised liquid chromatography with tandem mass spectrometry (LC-MS/MS) approach, and a data analytics pipeline with a comprehensive hazardous protein database. In addition, we developed a targeted MS assay for validating our screening results that confirmed the presence of some of selected hazardous proteins, and showed the quantification capability of this targeted assay at femtomole sensitivity.

INSTRUMENT(S):

ORGANISM(S): Gallus Gallus (chicken) Pisum Sativum (garden Pea) Solanum Tuberosum (potato) Fusarium Venenatum Vicia Faba Oryza Sativa (rice) Saccharomyces Cerevisiae (baker's Yeast) Glycine Max Triticum Aestivum (wheat)

SUBMITTER: Xiaowei Lou

LAB HEAD: Jayantha Gunaratne

PROVIDER: PXD056342 | Pride | 2025-07-07

REPOSITORIES: Pride

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			Action	DRS
	APBF1_Air-fried_SDS_DTT_R1.raw	Raw
	APBF1_Air-fried_SDS_DTT_R2.raw	Raw
	APBF1_Air-fried_SDS_DTT_R3.raw	Raw
	APBF1_Air-fried_SDS_R1.raw	Raw
	APBF1_Air-fried_SDS_R2.raw	Raw

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