Elucidating the Roles of TTP and SMAD2/3 in HEK293T through LC-MS Analysis

ABSTRACT: To further investigate the mechanism underlying the transcriptional regulation of m6A modification associated genes by TTP. we performed immunoprecipitation using anti-FLAG antibody in HEK293T cells transfected with pHAGE-TTP plasmids (overexpression of TTP) and then used mass spectrometry to identify the potential binding partners of TTP. the interactions between TTP and SMAD2/3 were confirmed by using coimmunoprecipitation in HEK293T, The N-Zn and Zn-C domain of TTP was found to be pivotal for its interaction with SMAD2/3.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: rui zhou  

LAB HEAD: rui zhou

PROVIDER: PXD056357 | Pride | 2026-05-14

REPOSITORIES: Pride