Dataset Information

Teleost spermatozoa can conduct de novo cytoplasmic and mitochondrial translation during post-testicular maturation

ABSTRACT: Translational silence of spermatozoa has long been considered the norm in animals. However, studies in mammals have shown that the mitochondrial ribosomal machinery is selectively activated during capacitation in the female reproductive tract, while cytoplasmic ribosomes remain inactive. Here, using quantitative proteomics in a piscine model species, we show that proteins involved in mRNA processing and cytoplasmic translation are predominantly accumulated in maturing spermatozoa within the extratesticular excurrent ducts, while those related to flagellar motility are enriched in mature (ejaculated) sperm. Based upon in vitro incubation of spermatozoa, motility assays and polysome profiling, we further show that 80S cytoplasmic and 55S mitochondrial ribosomes are actively involved in the translation of motility- and osmoadaptation-related proteins. These findings thus reveal that piscine spermatozoa can maintain de novo protein synthesis through both mitochondrial and cytoplasmic ribosomal activity during post-testicular maturation, which is necessary for the acquisition of full sperm function.

INSTRUMENT(S): LTQ Orbitrap, Orbitrap Fusion

ORGANISM(S): Sparus Aurata

TISSUE(S): Testis, Sperm

SUBMITTER: Montserrat Carrascal

LAB HEAD: Joan Cerda

PROVIDER: PXD056370 | Pride | 2025-05-07

REPOSITORIES: Pride

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Post-testicular spermatozoa of a marine teleost can conduct <i>de novo</i> cytoplasmic and mitochondrial translation.

Castro-Arnau Júlia J Chauvigné François F González Asier A Finn Roderick Nigel RN Carrascal Montserrat M Cerdà Joan J

iScience 20241206 1

Translational silence of spermatozoa has long been considered the norm in animals. However, studies in mammals have shown that the mitochondrial ribosomal machinery is selectively activated during capacitation in the female reproductive tract, while cytosolic ribosomes remain inactive. Here, using quantitative proteomics in a piscine model species, we show that proteins involved in mRNA processing and cytoplasmic translation are predominantly accumulated in immature spermatozoa within the extrat ...[more]

PMID: 39801836

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