Human RCC Binding Protein in Prostate Cancer Cell Line DU145
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Epithelial Cell, Cell Culture
DISEASE(S): Prostate Adenocarcinoma
SUBMITTER: 
Lizhong Wang
LAB HEAD: Lizhong Wang
PROVIDER: PXD056404 | Pride | 2025-07-23
REPOSITORIES: Pride
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| RCC2.csv | Csv | |||
| RCC2.mgf | Mgf | |||
| RCC2.mzML | Mzml | |||
| checksum.txt | Txt | |||
| control.csv | Csv |
Items per page: 5 1 - 5 of 7 |
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Project description:The CD24 cell surface receptor promotes apoptosis in developing B cells, and we recently found that it induces B cells to release plasma membrane-derived, CD24-bearing microvesicles (MVs). Here we have performed a systematic characterization of B cell MVs released from WEHI-231 B lymphoma cells in response to CD24 stimulation. We found that B cells constitutively release MVs of approximately 120 nm, and that CD24 induces an increase in phosphatidylserine-positive MV release. RNA cargo was predominantly comprised of 5S rRNA, regardless of stimulation; however, CD24 caused a decrease in the incorporation of protein coding transcripts. The MV proteome was enriched with mitochondrial and metabolism-related proteins after CD24 stimulation; however, these changes were variable and could not be fully validated by western blotting. CD24-bearing MVs carried Siglec-2, CD63, IgM, and, unexpectedly, Ter-119, but not Siglec-G or MHC-II despite their presence on the cell surface. CD24 stimulation also induced changes in CD63 and IgM expression on MVs not mirrored by the changes in cell surface expression. Overall, the composition of these MVs suggests that they may be involved in releasing mitochondrial components in response to pro-apoptotic stress with changes to the surface receptors potentially altering the cell type(s) that interact with the MVs.
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