Project description:Label-free whole-cell proteomics. Dataset 1: 15-days low-dose treatment with EtOH, 15uM ACT, 5ug/ml CAP or the combination ACT CAP in HEK293 WT cells. Dataset 2: 3-days low dose treatment with EtOH or 15uM ACT in HEK293 Cas9 and ATP6AP1 knockout cells
Project description:Transcriptome analysis of total RNA samples from HEK293-PIGS-KO and HEK293-PIGS-UBE2J1-DKO cells. To check whether KO of UBE2J1 upregulates genes of GPI biosthesis pathway, we used microarrays to analyze gene expression change by KO of UBE2J1 and comfirmed that known GPI pathway genes are not changed by ERAD-deficiency.
Project description:AP1M1 is an obligatory member of the AP1 clatherin adaptor complex, important for cellular trafficking. In this study, we investigated the transcriptomic profiles of cells lines (HEK293 and U2OS) in presence and in absence of AP1M1.