Project description:Label-free whole-cell proteomics. Dataset 1: 15-days low-dose treatment with EtOH, 15uM ACT, 5ug/ml CAP or the combination ACT CAP in HEK293 WT cells. Dataset 2: 3-days low dose treatment with EtOH or 15uM ACT in HEK293 Cas9 and ATP6AP1 knockout cells
Project description:Transcriptome analysis of total RNA samples from HEK293-PIGS-KO and HEK293-PIGS-UBE2J1-DKO cells. To check whether KO of UBE2J1 upregulates genes of GPI biosthesis pathway, we used microarrays to analyze gene expression change by KO of UBE2J1 and comfirmed that known GPI pathway genes are not changed by ERAD-deficiency.
Project description:We profiled the global cellular proteome of WT, PFDN3 knockout, and PFDN4 knockout cells using label-free quantification (LFQ) mass spectrometry.