Dataset Information

Anaerobic synthetic microbial communities

ABSTRACT: Anaerobic microbial communities play a critical role in biogeochemical cycles through complex networks of interactions that influence community structure, stability, and functionality. This study investigates the metabolic interactions within synthetic communities composed of a cellulolytic bacterium (Ruminiclostridium cellulolyticum), a hydrogenotrophic methanogen (Methanospirillum hungatei), an acetoclastic methanogen (Methanosaeta concilii), and a sulfate-reducing bacterium (Desulfovibrio vulgaris). Through proteogenomic analysis and metabolic modeling, we quantified the metabolic interaction potential and metabolic resource overlap across bi-, tri-, and quad-cultures.

INSTRUMENT(S):

ORGANISM(S): Desulfovibrio Vulgaris Str. Hildenborough Methanospirillum Hungatei Jf-1 Clostridium Cellulolyticum (strain Atcc 35319 / Dsm 5812 / Jcm 6584 / H10) Methanothrix Soehngenii Gp6

TISSUE(S): Cell Culture

SUBMITTER: Dongyu Wang

LAB HEAD: Chongle Pan

PROVIDER: PXD056517 | Pride | 2025-09-22

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	PanC_020624_MS2_TMT_1_SF01.raw	Raw
	PanC_020624_MS2_TMT_1_SF01_Proteins.txt	Txt
	PanC_020624_MS2_TMT_1_SF02.raw	Raw
	PanC_020624_MS2_TMT_1_SF03.raw	Raw
	PanC_020624_MS2_TMT_1_SF03_Proteins.txt	Txt

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Publications

Higher-order microbial interactions revealed by comparative metabolic modeling of synthetic communities with varying species composition.

Wang Dongyu D Hunt Kristopher A KA Abrahamson Britt B Flinkstrom Zachary Z Tao Xuanyu X Tanner Ralph S RS DeLeόn Kara B KB Zhou Aifen A Zhou Jizhong J McInerney Michael J MJ Winkler Mari-Karoliina H MH Stahl David A DA Candry Pieter P Pan Chongle C

ISME communications 20250101 1

Understanding how microbial interactions scale with community complexity is key to microbiome engineering and ecological theory. This study investigates emergent metabolic behaviors in controlled in vitro synthetic anaerobic communities of two, three, or four species: cellulolytic bacterium (Ruminiclostridium cellulolyticum), a hydrogenotrophic methanogen (Methanospirillum hungatei), an acetoclastic methanogen (Methanosaeta concilii), and a sulfate-reducing bacterium ...[more]

PMID: 40937471

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