Dataset Information

Nerve activity inhibits mTORC1-dependent protein synthesis in skeletal muscle

ABSTRACT: To find out intracellular signaling pathways regulting fiber type specific muscle wasting we generated a new mouse model, which allows us to label specifically muscle proteins. Interestingly, we observed that loss of muscle activity by denervation induces a strong increase of protein labeling in oxidative, mitochondria-rich muscle fibers. MS-based analysis, rapamycin treatments and genetic models showed requirement of mTORC1 signaling for increased protein synthesis in dependence of nerve activity.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Leg Muscle

SUBMITTER: Frederik Tellkamp

LAB HEAD: Bert Blaauw

PROVIDER: PXD056544 | Pride | 2025-05-07

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	202200812_Q3_LC6colID478_LK_MetRS_muscles_119_Gast.raw	Raw
	202200812_Q3_LC6colID478_LK_MetRS_muscles_119_TA.raw	Raw
	202200812_Q3_LC6colID478_LK_MetRS_muscles_120_Gast.raw	Raw
	202200812_Q3_LC6colID478_LK_MetRS_muscles_120_TA.raw	Raw
	202200812_Q3_LC6colID478_LK_MetRS_muscles_121_Gast.raw	Raw

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Publications

Neural stimulation suppresses mTORC1-mediated protein synthesis in skeletal muscle.

Dumitras Ana G AG Piccoli Giorgia G Tellkamp Frederik F Keufgens Lena L Baraldo Martina M Zorzato Sabrina S Cussonneau Laura L Nogara Leonardo L Krüger Marcus M Blaauw Bert B

Science advances 20250402 14

Skeletal muscle fibers are classified as glycolytic or oxidative, with differing susceptibilities to muscle wasting. However, the intracellular signaling pathways regulating fiber-specific muscle trophism remain unclear because of a lack of experimental models measuring protein synthesis. We developed a mouse model overexpressing a mutated transfer RNA synthetase in muscle fibers, enabling specific protein labeling using an artificial methionine substitute, which can be revealed through click ch ...[more]

PMID: 40173236

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