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The structural scaffold of the TPLATE complex deforms the membrane during plant endocytosis

ABSTRACT: Eukaryotic cells maintain homeostasis of their outer membrane by controlled internalization of lipid and protein constituents by endocytosis (Kaksonen & Roux, Nat Rev Mol Cell Biol. 2018). Endocytosis is evolutionary conserved and utilizes similar structural folds. How these structural folds are combined into proteins and protein complexes however differs between eukaryotic kingdoms (Kraus, Pleskot & Van Damme, Ann review 2024). The TPLATE complex in plants is an evolutionary ancient protein module that combines several endocytic folds into a single octameric protein complex (Gadeyne et al., Cell 2014, Hirst et al., eLife 2014, More et al., Curr Biol. 2020). Its molecular architecture, lipid-nucleated condensate formation, and its requirement for clathrin cage curvature revealed its function in endocytosis initiation in plants (Yperman & Wang et al., Sci Adv. 2021, Dragwidge et al., Nat Cell Biol. 2024, Johnson et al., PNAS 2021). Mechanistic understanding of how this complex drives membrane deformation during plant endocytosis is, however, lacking. Here, we used an integrative structural approach to obtain a precise molecular structure of the TPLATE complex. In addition, our approach also allowed visualizing the structural flexibility that hallmarks this enigmatic complex. We prove that the intrinsic structural flexibility is required for its functionality and membrane recruitment. We map different lipid-binding preferences onto specific domains located at the curved face of the complex. Finally, we show that the crescent shape of the structured part of the complex is sufficient for membrane curvature generation. Our mechanistic insight answers the longstanding question of how plants can execute endocytosis without cytoskeletal-based force generation.

INSTRUMENT(S):

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Cell Culture

SUBMITTER: Dominique Eeckhout

LAB HEAD: Daniel Van Damme

PROVIDER: PXD056680 | Pride | 2025-11-06

REPOSITORIES: Pride

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			Action	DRS
	Araport11plus_DE2020_1.fasta	Fasta
	Araport11plus_mikra_24012023.fasta	Fasta
	E30932_1p_Ionopticks4_trap19_PSB-639_1.raw	Raw
	E30934_1p_Ionopticks4_trap19_PSB-639_2.raw	Raw
	E30936_1p_Ionopticks4_trap19_PSB-639_3.raw	Raw

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The structural scaffold of the TPLATE complex deforms the membrane during plant endocytosis

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