Dataset Information

Pig myocardial tissue quantitative proteomics

ABSTRACT: Proteomics study of the anthracycline-induced cardiotoxicity in in pigs with pre-existing left ventricular (LV) pressure overload. Large White pigs (2-month-old males and females) were used to induce LV pressure overload via supravalvular aortic stenosis (or sham operation). After 4 months, animals were subsequently exposed to low-risk cumulative doses of anthracyclines (doxorubicin, 5 weekly 1 mg/kg intravenous injections) or vehicle. A total of 16 pigs were divided in four groups: (1) healthy controls (no LV overload or anthracyclines), (2) Doxorubicin (Dox, no LV overload but anthracyclines), (3) Banding (LV overload without anthracyclines), and (4) Banding+Doxorubicin (LV overload plus anthracyclines). A high-throughput quantitative proteomic analysis was performed in myocardial tissue.

INSTRUMENT(S):

ORGANISM(S): Sus Scrofa Domesticus (domestic Pig)

TISSUE(S): Heart

DISEASE(S): Cardiovascular System Disease

SUBMITTER: Inmaculada Jorge

LAB HEAD: Jesús María Vázquez Cobos

PROVIDER: PXD056684 | Pride | 2026-02-05

REPOSITORIES: Pride

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Publications

Anthracycline cardiotoxicity: role of metabolic vulnerability induced by cardiac pressure overload.

Galán-Arriola Carlos C Pérez-Camargo Daniel D Jorge Inmaculada I Bautista Víctor V Ayaon-Albarrán Ali A Pérez-Martínez Claudia C de Molina-Iracheta Antonio A Cádiz Laura L Medina-Hernández Danielle D Caballero-Henares Carlos C Lopez-Martín Gonzalo J GJ Vázquez Jesús J Ochala Julien J Fuster Valentin V Sánchez-González Javier J Ibáñez Borja B

European heart journal 20260113

<h4>Background and aims</h4>Hypertension and valvular heart disease, both associated with left ventricular (LV) pressure overload, increase the risk of anthracycline cardiotoxicity. While epidemiologically established, the underlying mechanisms remain unclear, precluding identification of therapeutic targets.<h4>Methods</h4>Two-month-old Yucatan pigs (males and females) underwent aortic banding to induce LV pressure overload or no operation. After 4 months, animals received a low-risk cumulative ...[more]

PMID: 41528064

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Project description:Background: Right ventricular (RV) and left ventricular (LV) myocardium differ in their response to pressure-overload hypertrophy (POH). In this report we use microarray and proteomic analyses to identify pathways modulated by LV-, and RV-POH in the immature heart. Methods: Newborn New Zealand White rabbits underwent banding of the descending thoracic aorta (LV-POH; n=6). RV-POH was achieved by banding the pulmonary artery (n=6). Sham–control animals (SC; n=6 each) were sham-manipulated. Following 4 (LV-POH) and 6 weeks (RV-POH) recovery, the hearts were removed and matched sample RNA and proteins were isolated for microarray and proteomic analysis. Results: There was no difference in body weight in RV-, LV-POH vs. SC but there was a significant increase vs. SC in RV (3.2±0.8g vs. 1.2±0.3g; P<0.01) and LV weight (7.08±0.6g vs. 4.02±0.2g; P<0.01). Fractional area change (RV-POH) and shortening fraction (LV-POH) decreased significantly (23±6 vs. 47±6 and 21±4 vs.44±2, respectively, P<0.01). Microarray analysis demonstrated that LV-POH enriched pathways for oxidative phosphorylation, mitochondria energy pathways, actin, ILK, hypoxia, calcium and protein kinase-A signalling. RV-POH enriched pathways for cardiac oxidative phosphorylation. Proteomic analysis revealed 19 proteins were uniquely expressed in LV-POH vs. SC. Functional annotation clustering analysis indicated significant enrichment for the mitochondrion, cellular macromolecular complex assembly and oxidative phosphorylation. RV-POH had 15 uniquely expressed proteins vs. SC. Functional annotation clustering analysis indicated significant enrichment in structural constituents of muscle, cardiac muscle tissue development and calcium handling. Conclusion: Our results identify unique transcript and protein expression profiles in LV, RV-POH and provide new insight into the biological basis of ventricular specific hypertrophy. 3 different conditions: PAB-RV vs. Sham-control RV, PAB-RV [test] vs. PAB-LV [control], AOB-LV vs. Sham-control LV.

			Action	DRS
	TMT1_BandigDoxo_new2.msf	Msf
	TMT1_BandigDoxo_new2.raw	Raw
	TMT1_BandingDoxo_new.msf	Msf
	TMT1_BandingDoxo_new.raw	Raw
	TMT2_BandigDoxo_new2.msf	Msf