Temporal and thermal optimization of trypsin digestion for the cryptococcal proteome

ABSTRACT: Mass spectrometry-based proteomics supports investigation into diverse biological systems, providing insights into regulation, signaling, modifications, and interactions. Optimized sample preparation is critical to ensure robust and reproducible analyses balanced with time and cost. In this article, we evaluated trypsin digestion parameters for the human fungal pathogen, Cryptococcus neoformans, and determined conditions for optimal peptide identification

INSTRUMENT(S):

ORGANISM(S): Cryptococcus Neoformans Var. Grubii Serotype A (strain H99 / Atcc 208821 / Cbs 10515 / Fgsc 9487) (filobasidiella Neoformans Var. Grubii)

SUBMITTER: Jennifer Geddes-McAlister  

LAB HEAD: Jennifer Geddes-McAlister

PROVIDER: PXD056755 | Pride | 2024-10-12

REPOSITORIES: Pride