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Chemical and photo-Crosslinking experiments of the BAX/BNIP3/PeptideB interaction

ABSTRACT: In these experiments we try to show by cross-linking MS how BAX and BNIP3 interact (DSSO cross-linking) and how Peptide B (an octapeptide that prevents mitochondrial damage and cell death induced by BNIP3/BAX activity) interacts with the BNIP3/BAX complex (BPA cross-linking; BPA on Peptide B).

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ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Farnusch Kaschani

LAB HEAD: Farnusch Kaschani

PROVIDER: PXD056758 | Pride | 2026-05-22

REPOSITORIES: Pride

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Project description:In this study, RNA-Seq was used to reveal the differences of molecular pathways in hepatopancreas of O. niloticus adapated to water with salinity of 8 or 16 practical salinity (psu), respectively, with fish at freshwater as the control,. Significantly changed pathways were mainly related to lipid metabolism, glucose utilization, protein consumption, osmotic regulation, signal transduction and immunology. Based on the tendencies from freshwater to 8 or 16 psu, the differentially expressed gene unions were categorized into eight unique models, which were further classified into three categories which were constant-change (either keep increasing or decreasing), change-then-stable and stable-then-change. In constant-change category, steroid biosynthesis, steroid hormone biosynthesis, fat digestion and absorption, complement and coagulation cascades were extremely significantly affected by ambient salinity (P < 0.01), indicating that these pathways play pivotal roles in molecular response to salinity acclimation from freshwater to saline water in O. niloticus, and should be the main research focus in the future. In change-then-stable category, ribosome, oxidative phosphorylation, peroxisome proliferator-activated receptors (PPAR) signaling pathway, fat digestion and absorption changed significantly with ambient increasing salinity (P < 0.01), showing these pathways were sensitive to environmental salinity variation, but had a response threshold, and would stop changing once salinity exceeds the threshold. In stable-then-change category, protein export, protein processing in endoplasmic reticulum, tight junction, thyroid hormone synthesis, antigen processing and presentation, glycolysis/gluconeogenesis and glycosaminoglycan biosynthesis - keratan sulfate were the top changed pathways (P < 0.01), suggesting that these pathways were not sensitive to salinity variation, but these pathways will respond significantly under salinity exceeding a certain level. The pathways and genes reported in this study laid on a solid foundation for future studies in understanding the underlying mechanism for salinity adaptation of freshwater fish. Examination of 3 different salinities treated hepatopancreas in Nile tilapia

			Action	DRS
	ACE_0836_CB02.raw	Raw
	ACE_0836_CB02a.raw	Raw
	ACE_0836_CB03.raw	Raw
	ACE_0836_CB03a.raw	Raw
	ACE_0836_SOI_MM_v01.fasta	Fasta

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Chemical and photo-Crosslinking experiments of the BAX/BNIP3/PeptideB interaction

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