Dataset Information

Identification and characterization of the elusive protein backbone of the immuno-dominant and specific Em2(G11) metacestode antigen of Echinococcus multilocularis

ABSTRACT: Alveolar echinococcosis (AE) caused by Echinococcus multilocularis is a severe zoonotic disease in humans. One of the major metacestode antigens of E. multilocularis is the Em2 or Em2(G11) native purified antigen. The main objective of the current study was to identify and characterize the elusive protein backbone of the specific and immuno-dominant Em2(G11) antigen of E. multilocularis. Two biological replicates were used. Following samples are submitted: European isolate (J2012) - Replicate 1 (Sample F284313): 20200127_08_J2012_1F.raw European isolate (J2012) - Replicate 2 (Sample F284314): 20200127_09_J2012_2F.raw Central Asian isolate (AT17) - Replicate 1 (Sample F284307): 20200127_10_AT17_1F.raw Central Asian isolate (AT17) - Replicate 2 (Sample F284308): 20200127_11_AT17_2F.raw

INSTRUMENT(S):

ORGANISM(S): Echinococcus Multilocularis

TISSUE(S): Metacestode

SUBMITTER: Ramon Eichenberger

LAB HEAD: Ramon M.

PROVIDER: PXD056760 | Pride | 2025-05-07

REPOSITORIES: Pride

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Identification and characterization of the elusive protein backbone of the immuno-dominant and species-specific Em2(G11) metacestode antigen of Echinococcus multilocularis.

Kronenberg Philipp A PA Laurimäe Teivi T Reinehr Michael M Deibel Ansgar A Hasler Sina S Gehrig Peter P Weber Achim A Deplazes Peter P Eichenberger Ramon M RM

Frontiers in parasitology 20250311

Alveolar echinococcosis (AE) caused by Echinococcus multilocularis, is a severe zoonotic disease in humans. One of the major metacestode antigens of E. multilocularis is the Em2 or Em2(G11) native purified antigen. The Em2 antigen is used for the serological and histopathological diagnosis of AE in humans and plays an important role in parasite-host interactions. As the Em2(G11) antigen is a mucin-type and glycosylated protein, the protein backbone has not been identified yet. We h ...[more]

PMID: 40135073

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Project description:The cestodes Echinococcus granulosus and Echinococcus multilocularis, as the pathogens of cystic echinococcosis and alveolar echinococcosis respectively, can cause significant health problems to the host and considerable socio-economic losses as a consequence. Based on the genomic data regarding these two species available in public database recently, we carried out high-throughput mRNA and small RNA transcriptomic sequencing of them and generate enormous transcriptomic datasets. A total of 34,717,856 reads (79.79%) mapped to E. granulosus genome, and 38,882,179 reads (87.61%) mapped to E. multiloculari genome. A total of 24,550 (7,925 known and 16,625 novel transcripts) and 23,771 transcripts (8,432 known and 15,339 novel transcripts) were assembled for E. granulosus and E. multilocularis respectively, and the assembly yielded 11,330 genes (6,815 known and 4,515 novel genes) for E. granulosus and 10,101 genes (7,051 known and 3,050 novel genes) for E. multilocularis, compared with the reference genome data. Bioinformatic analysis identified 6,826 AS events from 3,774 E. granulosus genes (33.31%) and 6,644 AS events in 3,611 E. multilocularis genes (35.75%). A total of 76,674 distinct microRNAs of E. granulosus and 115,742 of E. multilocularis were also obtained from small RNA transcriptome sequencing reads. Of these, there were 20 microRNAs of E. granulosus and 22 microRNAs of E. multilocularis that belonged to 19 and 21 microRNA families common to other metazoan lineages separately. 76 and 90 novel microRNAs so far unique to E. granulosus and E. multilocularis were also identified respectively. This study represents an extensive mRNA and small RNA transcriptome dataset obtained from the deep sequencing of these two cestode species. The findings will facilitate a more fundamental understanding of cestode biology, evolution, the host-parasite interplay, and provide new insights into the pathophysiology of echinococcosis, contributing to the development of improved interventions for disease control.

Project description:For Samples GSM601017-28: The aim of this study was to use expression profiling to define transcriptional patterns and regulatory pathways that characterize the host liver response to E. multilocularis infection in the experimental model of secondary infection, to compare gene expression in this model to those described in the model of "primary infection", and to follow the changes in gene expression and in the expression of a cell proliferation marker over time during the complete chronic phase of E.multilocularis infection, following early and middle stages. For Samples GSM601029-40: The aim of this study was to use expression profiling to define transcriptional patterns and regulatory pathways that characterize the host liver response to E. multilocularis infection in the experimental model of secondary infection, to compare gene expression in this model to those described in the model of "primary infection", and to follow the changes in gene expression and in the expression of a cell proliferation marker over time during the complete chronic phase of E.multilocularis infection, following its middle and late stages. For Samples GSM601017-28: The anterior liver tissue sample of control mice group and alveolar echinococcosis mice group were obtained at each time point(1 month and 3 month). Biological replicates is 3. Then, total RNA from anterior liver of both groups were used as test sample, which labelled cy5 fluorescein, and the pool RNA of healthy BALB/c mice anterior liver was used as reference sample, which labelled cy3 fluorescein, and then hybridized to 32K Mouse Genome Array Genechips, representing about 25000 characterized murine genes. For Samples GSM601029-40: The anterior liver tissue sample of control mice group and alveolar echinococcosis mice group were obtained at each time point(2 month and 6 month). Biological replicates is 3. Then, total RNA from anterior liver of both groups were used as test sample, which labelled cy5 fluorescein, and the pool RNA of healthy BALB/c mice anterior liver was used as reference sample, which labelled cy3 fluorescein, and then hybridized to 36K Mouse Genome Array Genechips, representing about 25000 characterized murine genes.

			Action	DRS
	20200127_08_J2012_1F.raw	Raw
	20200127_09_J2012_2F.raw	Raw
	20200127_10_AT17_1F.raw	Raw
	20200127_11_AT17_2F.raw	Raw
	F284307.dat	Other

Dataset Information

Identification and characterization of the elusive protein backbone of the immuno-dominant and specific Em2(G11) metacestode antigen of Echinococcus multilocularis

Dataset's files

Publications

Identification and characterization of the elusive protein backbone of the immuno-dominant and species-specific Em2(G11) metacestode antigen of <i>Echinococcus multilocularis</i>.

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OmicsDI is part of the ELIXIR infrastructure

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Dataset Information

Identification and characterization of the elusive protein backbone of the immuno-dominant and specific Em2(G11) metacestode antigen of Echinococcus multilocularis

Dataset's files

Publications

Identification and characterization of the elusive protein backbone of the immuno-dominant and species-specific Em2(G11) metacestode antigen of &lt;i&gt;Echinococcus multilocularis&lt;/i&gt;.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Identification and characterization of the elusive protein backbone of the immuno-dominant and species-specific Em2(G11) metacestode antigen of <i>Echinococcus multilocularis</i>.