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Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neurons

ABSTRACT: Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neurons. Lysosomes are membrane-bound organelles critical for maintaining cellular homeostasis. Delivery of biosynthetic lysosomal proteins to lysosomes is crucial to orchestrate proper lysosomal function. However, it remains unknown how the delivery of biosynthetic lysosomal proteins to lysosomes is ensured in neurons, which are highly polarized cells. Here, we developed Protein Origin, Trafficking And Targeting to Organelle Mapping (POTATOMap), by combining trafficking synchronization and proximity-labelling based proteomics, to unravel the trafficking routes and interactome of the biosynthetic lysosomal membrane protein LAMP1 at specified time points.

INSTRUMENT(S):

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Primary Cell, Neuron

SUBMITTER: Chun Hei Li

LAB HEAD: Ginny G. Farias

PROVIDER: PXD056787 | Pride | 2025-02-27

REPOSITORIES: Pride

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			Action	DRS
	20230525_EX3_UM1_Li000149_SA_EXT02_0H_neg_A1.raw	Raw
	20230525_EX3_UM1_Li000149_SA_EXT02_0H_neg_A2.raw	Raw
	20230525_EX3_UM1_Li000149_SA_EXT02_0H_neg_B1.raw	Raw
	20230525_EX3_UM1_Li000149_SA_EXT02_0H_neg_B2.raw	Raw
	20230525_EX3_UM1_Li000149_SA_EXT02_0H_pos_A1.raw	Raw

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Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neurons

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