Dataset Information

Melatonin confers saline-alkali tolerance by relieving nitrosative damage and S-nitrosylation of plasma membrane H+-ATPase 2 in tomato

ABSTRACT: Soil salination and alkalization are global problems impairing plant survival by disrupting REDOX homeostasis. Whether melatonin regulates REDOX homeostasis at nitrosative level, and thus affects plant saline-alkali tolerance remains unknown. In saline-alkali stress, excess nitric oxide (NO) causes nitrosative damage in tomato roots. This NO can be degraded by S-nitrosoglutathione reductase (GSNOR), or stimulates caffeic acid O-methyltransferase (COMT) transcript for melatonin synthesis. Melatonin further feedback scavenges excess NO to alleviate nitrosative damage at the whole protein level, indicating by proteome S-nitrosylation. We target plasma membrane H+-ATPase 2 (HA2) and highlight that HA2 is S-nitrosylated at Cys206 in saline-alkali stress, reducing HA activity, H+ efflux, and tolerance by impairing its interaction with 14-3-3 protein 1 (TFT1). In agreement with these observations, COMT-mediated melatonin relieves the HA2 S-nitrosylation to recover its function and saline-alkali tolerance. Therefore, we propose NO and melatonin as a pair of REDOX switches to control HA2 S-nitrosylation and saline-alkali tolerance. Under natural saline-alkali conditions, tomato productivity can be improved by grafting with COMT-, GSNOR-, HA2-overexpression rootstocks or by genetic engineering non-nitrosylated HA2C206S mutants. Using melatonin-NO-HA2 module as a case, this study illuminates a novel molecular function of melatonin and relevant genetic engineering strategies in future agriculture.

INSTRUMENT(S): orbitrap

ORGANISM(S): 'solanum Lycopersicum' Phytoplasma

TISSUE(S): Root

SUBMITTER: guochen qin

LAB HEAD: guochen qin

PROVIDER: PXD056815 | Pride | 2025-04-28

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	P-SN-GB-tomato-root-TMT.pdResult	Other
	P-SN-GB-tomato-root-TMT.raw	Raw

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Publications

Melatonin confers saline-alkali tolerance in tomato by alleviating nitrosative damage and S-nitrosylation of H+-ATPase 2.

Wei Jin-Wei JW Liu Minghui M Zhao Dan D Du Pengmeng P Yan Lu L Liu Derui D Shi Qinghua Q Yang Changxian C Qin Guochen G Gong Biao B

The Plant cell 20250201 2

Soil salinization and alkalization disrupt redox homeostasis, impairing plant survival and crop production. Disruption of redox homeostasis can cause accumulation of reactive nitrogen species, such as nitric oxide (NO), which causes nitrosative damage in which the properties of biomacromolecules are altered. It is unclear whether melatonin regulates NO homeostasis, thereby affecting plant saline-alkali tolerance. In tomato (Solanum lycopersicum), excess NO caused by saline-alkali stress resulted ...[more]

PMID: 39928568

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