An Nlrp5-null mutation leads to attenuated de novo methylation in oocytes, accompanied by significant reduction in DNMT3L

ABSTRACT: Nlrp5 encodes a core component of the subcortical maternal complex (SCMC) a cytoplasmic protein structure unique to the mammalian oocyte and cleavage-stage embryo. NLRP5 mutations have been identified in patients presenting with early embryo arrest, recurrent molar pregnancies and imprinting disorders. It was previously shown that oocytes with mutations in the human SCMC gene KHDC3L had globally impaired methylation, suggesting a requirement for integrity of the SCMC in the establishment of DNA methylation. Here, we present a multi-omic analysis of an Nlrp5-null mouse model. We detect global misregulation of maternal proteins, including profound reduction in the essential de novo methylation factor DNMT3L, which could contribute to attenuated de novo methylation we observe. In contrast, the maintenance methyltransferase DNMT1 exhibits normal cytoplasmic localisation. This provides evidence for mechanisms leading to downstream misregulation of imprinted genes, which in turn, may result in imprinting syndromes, multi-locus imprinting disturbances (MLID) and hydatidiform moles.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Oocyte

SUBMITTER: David Oxley  

LAB HEAD: david oxley

PROVIDER: PXD056859 | Pride | 2025-12-22

REPOSITORIES: Pride