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Comparative proteomic analysis of self-compatible Turnera mutants suggests Glutathione S-transferase 8 is involved in overcoming S-morph female self-incompatibility response. Part 2 Turnera scabra mutant vs WT analysis

ABSTRACT: Comparative proteomic analysis of self-compatible mutant S-morph Turnera scabra vs WT S-morph T. scabra

INSTRUMENT(S):

ORGANISM(S): Turnera Scabra

TISSUE(S): Pollen

SUBMITTER: Paige Henning

LAB HEAD: Paige M Henning

PROVIDER: PXD057031 | Pride | 2025-12-08

REPOSITORIES: Pride

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Comparative Proteomic Analysis of Self-Compatible Turnera Mutants Suggests Glutathione S-Transferase 8 Is Involved in Overcoming S-Morph Female Self-Incompatibility Response.

Henning Paige M PM Chafe Paul D J PDJ Hamam Hasan J HJ Shore Joel S JS

Plant direct 20251128 12

Distyly is a reproductive system, characterized by the presence of two floral morphs, which promotes outcrossing via physical and biochemical means. In distylous Turnera, the mating type of the S-morph is determined by two genes: YUC6 (male) and BAHD (female). Despite the importance of these S-genes, it is likely that additional genes are involved in the distylous syndrome. Here, we use comparative mass spectrometry analysis to identify differentially expressed protei ...[more]

PMID: 41321591

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Project description:Heat stress is a major cause for yield loss in many crops, including vegetable crops. Even short waves of high temperature, becoming more frequent during recent years, can be detrimental. Pollen development is most heat-sensitive, being the main cause for reduced productivity under heat-stress across a wide range of crops. The molecular mechanisms involved in pollen heat-stress response and thermotolerance are however not fully understood. Recently, we have demonstrated that ethylene, a gaseous plant hormone, plays a role in tomato (Solanum lycopersicum) pollen thermotolerance. These results were substantiated in the current work showing that increasing ethylene levels by using an ethylene-releasing substance, ethephon, prior to heat-stress exposure, increased pollen quality. A proteomic approach was undertaken, to unravel the mechanisms underlying pollen heat-stress response and ethylene-mediated pollen thermotolerance in developing pollen grains. Proteins were extracted and analyzed by means of a gel LC-MS fractionation protocol, and a total of 1355 proteins were identified. A dataset of 721 proteins, detected in three biological replicates of at least one of the applied treatments, was used for all analyses. Quantitative analysis was performed based on peptide count. The analysis revealed that heat-stress affected the developmental program of pollen, including protein homeostasis (components of the translational and degradation machinery), carbohydrate and energy metabolism. Ethephon-pretreatment shifted the heat-stressed pollen proteome closer to the proteome under non-stressful conditions, namely, by showing higher abundance of proteins involved in protein synthesis, degradation, tricarboxylic acid cycle and RNA regulation. Furthermore, up-regulation of protective mechanisms against oxidative stress was observed following ethephon-treatment (including higher abundance of glutathione-disulfide reductase, glutaredoxin and protein disulfide isomerase). Taken together, the findings identified systemic and fundamental components of pollen thermotolerance, and serve as a valuable quantitative protein database for further research.

Project description:The formation of a zygote by the fusion of egg and sperm involves the two gametic transcriptomes. In flowering plants, the embryo sac embedded within the ovule contains the egg cell, while the pollen grain contains two sperm cells inside a supporting vegetative cell. The difficulties of collecting isolated gametes and consequent low recovery of RNA have restricted in-depth analysis of gametic transcriptomes in flowering plants. We isolated living egg cells, sperm cells, and pollen vegetative cells from rice, and identified transcripts for ~36,000 genes by deep sequencing. The three transcriptomes are highly divergent, with about three quarters of those genes differentially expressed in the different cell types. Distinctive expression profiles were observed for genes involved in chromatin conformation, including an unexpected expression in the sperm cell of genes associated with active chromatin. Furthermore, both the sperm cell and the pollen vegetative cell were deficient in expression of key RNAi components. Differences in gene expression were also observed for genes for hormonal signaling and cell cycle regulation. The egg cell and sperm cell transcriptomes reveal major differences in gene expression to be resolved in the zygote, including pathways affecting chromatin configuration, hormones and cell cycle. The sex-specific differences in expression of RNAi components suggest that epigenetic silencing in the zygote might act predominantly through female-dependent pathways. More generally, this study provides a detailed gene expression landscape for flowering plant gametes, enabling the identification of specific gametic functions and their contributions to zygote and seed development. The gene expression profiles of the three gametic cells, egg cell (EC), sperm cell (Sp) and vegetative cell (Ve) were compared via RNA-seq using three biological replicates for each. Differential expression (DE) analysis was performed using edgeR and the resulting DE genes were assessed for Gene Ontology term enrichment.

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	TScabra_Mutantrep1.raw	Raw
	TScabra_Mutantrep2.raw	Raw
	TScabra_Mutantrep3.raw	Raw
	TScabra_Mutantrep4.raw	Raw
	TScabra_Mutantrep5.raw	Raw

Dataset Information

Comparative proteomic analysis of self-compatible Turnera mutants suggests Glutathione S-transferase 8 is involved in overcoming S-morph female self-incompatibility response. Part 2 Turnera scabra mutant vs WT analysis

Dataset's files

Publications

Comparative Proteomic Analysis of Self-Compatible <i>Turnera</i> Mutants Suggests <i>Glutathione S-Transferase 8</i> Is Involved in Overcoming S-Morph Female Self-Incompatibility Response.

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Dataset Information

Comparative proteomic analysis of self-compatible Turnera mutants suggests Glutathione S-transferase 8 is involved in overcoming S-morph female self-incompatibility response. Part 2 Turnera scabra mutant vs WT analysis

Dataset's files

Publications

Comparative Proteomic Analysis of Self-Compatible &lt;i&gt;Turnera&lt;/i&gt; Mutants Suggests &lt;i&gt;Glutathione S-Transferase 8&lt;/i&gt; Is Involved in Overcoming S-Morph Female Self-Incompatibility Response.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Comparative Proteomic Analysis of Self-Compatible <i>Turnera</i> Mutants Suggests <i>Glutathione S-Transferase 8</i> Is Involved in Overcoming S-Morph Female Self-Incompatibility Response.