Dataset Information

Arabidopsis AGO1 interactome under Cu deficiency and Cu sufficiency

ABSTRACT: In this project we look for AGO1 protein interactors in pAGO1-TST-AGO1 (Col-0) transgenic plants grown under Cu deficiency or under cu sufficiency. TST-AGO1 complexes were affinity purified and proteins were identified by tandem MS.

INSTRUMENT(S):

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Seedling

SUBMITTER: Alberto Carbonell

LAB HEAD: Alberto Carbonell

PROVIDER: PXD057108 | Pride | 2025-11-17

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	AGO-sequences.fasta	Fasta
	AlbertoCarbonell_sol401-41-21_AC-MS-2_09062021.msf	Msf
	AlbertoCarbonell_sol401-41-21_AC-MS-2_09062021.raw	Raw
	AlbertoCarbonell_sol401-41-21_AC-MS-3_09062021.msf	Msf
	AlbertoCarbonell_sol401-41-21_AC-MS-3_09062021.raw	Raw

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Publications

Arabidopsis thaliana iron superoxide dismutase FeSOD1 protects ARGONAUTE 1 in a copper-dependent manner.

Tomassi Ariel H AH Perea-García Ana A Rodrigo Guillermo G Sánchez-Vicente Javier J Cisneros Adriana E AE Olmos Marta M Andrés-Bordería Amparo A López-Dolz Lucio L Daròs José-Antonio JA Peñarrubia Lola L Carbonell Alberto A

Journal of experimental botany 20251101 18

Copper (Cu) deficiency compromises plant growth and limits crop productivity. Plants respond to Cu scarcity by activating the expression of several miRNAs, known as Cu-miRNAs, which degrade mRNAs from various cuproproteins to conserve Cu. Cu-miRNAs, like most plant miRNAs, associate with ARGONAUTE 1 (AGO1), the primary effector protein of miRNA-mediated gene silencing pathways, whose function is typically modulated by interacting proteins acting as cofactors. However, how AGO1 is regulated and f ...[more]

PMID: 40576540

Similar Datasets

Project description:The transition metal copper (Cu) is an essential element for all living organisms. In plants, Cu plays key roles in electron transport chains of chloroplasts and mitochondria, as well as in cell wall metabolism, ethylene perception, molybdenum cofactor synthesis and oxidative stress protection. Because of its physiological importance, suboptimal concentrations of Cu trigger a re-organization of metabolism to economize on Cu, and pronounced Cu deficiency causes severe growth reduction and defects in male fertility. However, when present in excess, Cu can be highly toxic. Therefore, Cu uptake, utilization and cellular concentrations are strictly regulated. A number of components of the Cu-homeostatic network of Arabidopsis have already been identified. However, the mechanisms that control responses of plant gene expression to Cu-deficiency are only partly understood. In Chlamydomonas reinhardtii, the transcription factor Crr1 is required for activating and/or repressing the expression of a number of genes in response to Cu deficiency. This protein contains a plant-specific DNA-binding domain (SBP domain), ankyrin repeats and a C-terminal cysteine-rich region with similarity to a Drosophila metallothionein (MT). In Arabidopsis, there is a family of 16 proteins with SBP domains named SPL family (SBP-like) of which a subset of proteins have been implicated in flowering time control and floral development. Among all of them, AtSPL7 is the most similar to Crr1 (27 % identity), and AtSPL1 (25 % identity), AtSPL12 (24 % identity) and AtSPL14 (23 % identity) share a common protein architecture. The goal of this work was to obtain a complete account of the response of the Arabidopsis thaliana transcriptome to Cu deficiency, as well as to determine the role of AtSPL7 in the transcriptional response to Cu deficiency. For this purpose, three independent experiments were carried out including Col-0 wild-type and spl7-2 mutant plants grown in Cu-sufficient and Cu-deficient hydroponic media, respectively. Root and shoot transcriptomes were established by RNA-Seq for quantitative comparisons. Sampling of root and shoot tissues of wild-type (WT, Col-0) and spl7-2 mutant plants (the mutant is knock-down for the transcription factor SPL7, which plays a key role in the transcriptional regulation of the copper deficiency responses) cultivated hydroponically in a modified Hoagland's solution under Cu-sufficient (control) and Cu-deficient conditions.