Dataset Information

Proteomics analysis of E. coli expressing BamA variants

ABSTRACT: Here we present proteomics data for the analysis of E coli expressing BamA variants, with a LVPR insertion in the hinge region between the barrel and POTRA domains, or with the T434A mutation.

INSTRUMENT(S):

ORGANISM(S): Escherichia Coli

SUBMITTER: Antonio Calabrese

LAB HEAD: Antonio Calabrese

PROVIDER: PXD057225 | Pride | 2025-10-13

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	23118_Anton_Ecl01_DDA_IT50_TRAP_50cm_120min_T434A_01_01.raw	Raw
	23118_Anton_Ecl01_DDA_IT50_TRAP_50cm_120min_T434A_02_01.raw	Raw
	23118_Anton_Ecl01_DDA_IT50_TRAP_50cm_120min_T434A_03_01.raw	Raw
	23118_Anton_Ecl01_DDA_IT50_TRAP_50cm_120min_WT_01_01.raw	Raw
	23118_Anton_Ecl01_DDA_IT50_TRAP_50cm_120min_WT_02_01.raw	Raw

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Publications

Molecular insights into how the motions of the β-barrel and POTRA domains of BamA are coupled for efficient function.

Csoma Naemi N Machin Jonathan M JM Whitehouse James M JM Rodrìguez-Alonso Raquel R Olejnik Monika M Cahill Adam K AK Cho Seung-Hyun SH Schäberle Till F TF Iorga Bogdan I BI Ranson Neil A NA Radford Sheena E SE Calabrese Antonio N AN Collet Jean-François JF

Nature communications 20251003 1

The β-barrel assembly machinery (BAM) inserts β-barrel proteins into the outer membrane of Gram-negative bacteria, forming an essential permeability barrier. The core BAM component, BamA, is a β-barrel protein with an N-terminal periplasmic extension comprising five polypeptide transport-associated (POTRA) domains. Whilst BamA's structure is well characterised, it remains unclear how β-barrel and POTRA domain motions are coordinated. Using BamA variants with mutations in the hinge region between ...[more]

PMID: 41044071

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Project description:Onchocerca volvulus is the agent of onchocerciasis or river blindness and targeted by WHO for elimination. The main strategy is mass drug administration with ivermectin for the duration of the life span of the adult worms. The drug is mainly microfilaricidal, but weak macrofilaricidal effects, after several rounds of treatment, were observed. A small percentage of adult O. volvulus females contain pleomorphic neoplasms and the rate of worms with neoplasms increases with the number of ivermectin treatments. Within a clinical trial of drug combinations that included ivermectin we identified 24 (5.6%) adult females with neoplasms. We assessed the protein inventory of these neoplasms to identify proteins that may be associated with tumor development. We used laser capture microdissection and highly sensitive mass spectrometry analysis to determine the protein inventory of neoplasms. Neoplasm tissue from female worms was analyzed, and compared to normal tissue from the body wall, uterus and intestine from the same worms, and to tissues from females without neoplasms. One female with neoplasms had a few normal stretched microfilariae in the vagina, but no normal embryogenesis was seen in the other two worms. The healthy females showed all intact embryogenesis. In the worms with neoplasms, 151 proteins were detected in the body wall, 215 proteins in the intestine, 47 proteins in the uterus and 1,577 proteins in the neoplasms. Only in the uterus of the female with intrauterine microfilariae an elevated number of proteins (601) was detectable, while in the uteri of the healthy females 1,710 proteins were detected. The majority of the 20 most abundant proteins identified in neoplasms was highly conserved and only two nematode specific proteins were observed. Among the most abundant proteins that were found in neoplasms but not in the other analyzed tissues, were peroxiredoxin, proteases, and proteins related to signal transduction, ribosomal and proteasome activity. Immunolocalization of a calcium binding protein that was detected in neoplasms confirmed the mass spectrometry results. In conclusion, we have developed a system to analyze the proteome of O. volvulus from nodule sections and identified proteins that are potentially linked to the development of neoplasms and may contribute to worm mortality.