Dataset Information

Cleavage of Cartilage Oligomeric Matrix Protein (COMP) by ADAMTS4 generates a neoepitope associated with osteoarthritis severity and progression

ABSTRACT: The major proteoglycan in cartilage is COMP. This protein undergoes extensive proteolytic turnover that is believed to be dysregulated during osteoarthritis. Here we identify and describe two secreted proteases that are responsible for this turnover and identify the specific sites of proteolysis by each protease. This data was used to generate a neo-epitope antibody for validating these cleavage sites in human tissues.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

DISEASE(S): Osteoarthritis

SUBMITTER: Daniel Martin

LAB HEAD: Suneel Apte

PROVIDER: PXD057263 | Pride | 2025-02-26

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Lum_19may2008_COMP_TS1.msf	Msf
	Lum_19may2008_COMP_TS1.mzML	Mzml
	Lum_19may2008_COMP_TS1.mzid.gz	Mzid
	Lum_19may2008_COMP_TS1.raw	Raw
	Lum_19may2009_COMP_TS1EQ.raw	Raw

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Publications

Cleavage of Cartilage Oligomeric Matrix Protein (COMP) by ADAMTS4 generates a neoepitope associated with osteoarthritis and other forms of degenerative joint disease.

de Groot Rens R Folgado Patricia Badía PB Yamamoto Kazuhiro K Martin Daniel R DR Koch Christopher D CD Debruin Danielle D Blagg Sophie S Minns Alexander F AF Bhutada Sumit S Ahnström Josefin J Larkin Jonathan J Aspberg Anders A Önnerfjord Patrik P Apte Suneel S SS Santamaria Salvatore S

Matrix biology : journal of the International Society for Matrix Biology 20241211

Osteoarthritis (OA) is a highly prevalent joint disease, affecting millions of people worldwide and characterized by degradation of articular cartilage, subchondral bone remodeling and low-grade inflammation, leading to pain, stiffness and disability. Cartilage Oligomeric Matrix Protein (COMP) is a major structural component of cartilage and its degradation has been proposed as a marker of OA severity/progression. Several proteases cleave COMP in vitro, however, it is unclear which of these COMP ...[more]

PMID: 39672391

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