Proteomics

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Proteomics effects of exposure of fatty acids in the human liver cell line Huh7

ABSTRACT: Non-alcoholic fatty liver disease (NAFLD), also referred to metabolic associated fatty liver disease (MAFLD), displays characteristics indicative of metabolic disorders. Despite extensive research efforts, the underlying pathogenesis of NAFLD remain largely unknown and currently lack recommended treatment options. It is widely acknowledged that obesity and type 2 diabetes are significant risk factors for NAFLD, with the accumulation of free fatty acids (FFA) in the liver considered an initial trigger for its development. In this study, we exposed the human liver cell line Huh7 to a high concentration of a mixture containing both saturated (palmitic acid) and unsaturated (oleic acid) fatty acids for 24 hours. TMT and LC-MS based proteomics yielded a set of 172 significantly regulated proteins.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hepatocyte, Cell Culture

SUBMITTER: Johan Palmfeldt  

LAB HEAD: Johan Palmfeldt

PROVIDER: PXD057281 | Pride | 2026-03-16

REPOSITORIES: Pride

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Tian-ran-Huh7-merge20-2.msf Msf
X4202-Tian-ran-Huh7-1-1.raw Raw
X4202-Tian-ran-Huh7-1-1_20200725135928.raw Raw
X4204-Tian-ran-Huh7-1-2.raw Raw
X4204-Tian-ran-Huh7-1-2_20200725183634.raw Raw
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Publications

Energy metabolism alteration and gene expression reprogramming in a cell model of high fat load non-alcoholic fatty liver disease.

Zhou Tianran T   Zhou Yuhang Y   Cömert Cagla C   Zhou Xiao-Yu XY   Lin Lin L   Bolund Lars L   Palmfeldt Johan J   Luo Yonglun Y   Bross Peter P   Tong Guangdong G  

BMC gastroenterology 20260207 1

BACKGROUND: Metabolic rewiring plays a crucial role in the energy metabolism of hepatocytes during steatosis. However, the precise alterations in energy metabolism remain unclear. The aim of this study was to investigate the effects of lipid exposure on cellular energy metabolism and gene expression in a cellular model of non-alcoholic fatty liver disease (NAFLD). METHODS AND RESULTS: We induced hepatocyte steatosis in the hepatocyte cell line Huh7 via treatment with high levels of palmitate and  ...[more]

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