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Human LC-MS (ITCH-Mediated Golgi Fragmentation Drives Proteotoxicity in Neurodegeneration)

ABSTRACT: To identify host proteins that interact with ITCH, we performed proteomic analysis of the co-immunoprecipitates pulled down by the ITCH expressed in HEK293 cells.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Qiwang Xiang

LAB HEAD: Jiou Wang

PROVIDER: PXD057324 | Pride | 2025-09-19

REPOSITORIES: Pride

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			Action	DRS
	210427_WangJ_QX_S1_EV.raw	Raw
	210427_WangJ_QX_S2_IT-ubi.raw	Raw
	210427_WangJ_QX_S3_IT-CS.raw	Raw
	210427_WangJ_QX_S5_IT-ev.raw	Raw
	ITCH.txt	Txt

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Project description:We found that the E3 ubiquitin ligase Itch significantly affects early B-cell differentiation. To explore the role of Itch in late B-cell differentiation, we sorted B cells from WT and Itch KO mice. To explore the effect of Itch deficency on gene expression, we determined mRNA profiles in B cells from WT and Itch KO mice by RNA-seq. RNA-seq was done with an Illumina HiSeq 2500 instrument at GENEWIZ, Suzhou, China.

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