Proteomics

Dataset Information

0

TRNA as an assembly chaperone for a macromolecular transcription-processing complex

ABSTRACT: tRNAs are widely recognized for their role in translation. Here we describe a previously unidentified function of tRNA as an assembly chaperone. During poxviral infection tRNAGln/Arg lacking the anticodon mcm5s2U modification is specifically recruited from the cellular tRNA pool to a multi-subunit poxviral RNA polymerase complex (vRNAP), where it controls the transition to the pre-initiation complex upon infection. Cryo-EM analysis of assembly intermediates illustrates how tRNAGln/Arg orchestrates the recruitment of transcription and mRNA processing factors to vRNAP. The atomic vRNAP structures reveal an induced-fit mechanism that internalizes anticodon base G36 into the anticodon stem creating a non-canonical tRNA structure and selecting a defined tRNA modification pattern. The role of tRNA as assembly chaperone extends to the pathogenic MPXV due to the high conservation of vRNAP.

INSTRUMENT(S):

ORGANISM(S): Vaccinia Virus Glv-1h68 Homo Sapiens (human)

TISSUE(S): Hela-s3 Cell, Malignant Cell

SUBMITTER: Julian Bender  

LAB HEAD: Bettina Warscheid

PROVIDER: PXD057359 | Pride | 2025-11-24

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
QEplus034526.raw Raw
QEplus034527.raw Raw
QEplus034528.raw Raw
QEplus034531.raw Raw
QEplus034532.raw Raw
Items per page:
1 - 5 of 13
altmetric image

Publications

Transfer RNAs (tRNAs) are widely recognized for their role in translation. Here, we describe a previously unidentified function of tRNA as an assembly chaperone. During poxviral infection, tRNA<sup>Gln/Arg</sup> lacking the anticodon mcm<sup>5</sup>s<sup>2</sup>U34 modification is specifically sequestered from the cellular tRNA pool to promote formation of a multisubunit poxviral RNA polymerase complex (vRNAP). Cryo-electron microscopy analysis of assembly intermediates illustrates how tRNA<sup>  ...[more]

Similar Datasets

Transcriptomics RNA-seq mouse development
2014-08-08 | E-MTAB-2328 | biostudies-arrayexpress
Transcriptomics Optimization of codon translation rates via tRNA modifications maintains proteome integrity
2015-06-04 | E-GEOD-67387 | biostudies-arrayexpress
Transcriptomics Impact of tRNA-Glu(UUC) and tRNA-Arg(CCG) on mRNA abundance
2016-06-07 | GSE77285 | GEO
Transcriptomics Deciphering the reading of the genetic code by near-cognate tRNA
2018-04-04 | GSE108772 | GEO
Genomics Effect of trmA on tRNA expression in Pseudomonas aeruginosa
2025-06-08 | GSE252634 | GEO
Transcriptomics Impact of tRNA-Glu(UUC) and tRNA-Arg(CCG) on mRNA stability
2016-06-07 | GSE77349 | GEO
Proteomics Quantifying mistranslation from alanine tRNA anticodon variants in S. cerevisiae
2023-10-02 | PXD038242 | Pride
Transcriptomics Quantitative analysis of small RNA pseudouridylation reveals interplay of PUS enzymes in tRNA anticodon stem-loop
2025-11-27 | GSE299274 | GEO
Transcriptomics A Ψ–Ψ codon–anticodon pairing in nonsense suppression and translational recoding
2025-06-20 | GSE299290 | GEO
Genomics Cell type-specific expression of tRNAs in the brain regulates cellular homeostasis.
2024-02-19 | GSE250090 | GEO