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Proteomic analysis of hippocampal tissue from adult CMTR1-cKO mice

ABSTRACT: Eukaryotic mRNA is 5-end capped with m7 guanosine, forming the cap0 (m7GpppNpNp, N: any nucleotide). Cap methyltransferase (CMTR1) further catalyzes 2-O-ribose methylation of the first transcribed nucleotide (N1 2-O-Me) to produce the cap1 (m7GpppNmNp) structure in all eukaryotes except yeasts. To elucidate the molecular changes in the hippocampus caused by the deletion of CMTR1 in Emx1-lineage cells, hippocampal tissues from CMTR1-cWT and cKOEmx1 mice were processed for proteomic analysis.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Yi-Shuian Huang

LAB HEAD: Dr. Yi-Shuian Huang

PROVIDER: PXD057419 | Pride | 2025-08-29

REPOSITORIES: Pride

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			Action	DRS
	220117-TMT-40-55-65CV-A.raw	Raw
	220117-TMT-40-55-65CV-B.raw	Raw
	220117-TMT-40-55-65CV-C.raw	Raw
	220117-TMT-40-55-65CV-D.raw	Raw
	220117-TMT-40-55-65CV-E.raw	Raw

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Project description:Eukaryotic mRNA is 5'-end capped with m7 guanosine, forming the cap0 (m7GpppNpNp, N: any nucleotide). Cap methyltransferase (CMTR1) further catalyzes 2'-O-ribose methylation of the first transcribed nucleotide (N1 2'-O-Me) to produce the cap1 (m7GpppNmNp) structure in all eukaryotes except yeasts. To elucidate the molecular changes in the hippocampus caused by the deletion of CMTR1 in Emx1-lineage cells, hippocampal tissues from CMTR1-cWT and cKOEmx1 mice were processed for RNA-sequencing analysis.

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Part 2 JAWS; 2'-O-methylation of the second transcribed nucleotide in eukaryotic RNA 5’ cap impacts protein production levels and susceptibility to DXO-mediated transcript degradation

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