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What does next generation Mass Spectrometry offer for proteomics? A comprehensive platform comparison

ABSTRACT: Next-generation mass spectrometry platforms (Orbitrap Astral, timsTOF Ultra) are reshaping proteomics by enhancing analytical depth and sensitivity. We compared these platforms against Orbitrap Exploris 480 using neonatal mouse lung tissue from a bronchopulmonary dysplasia model (n=12), employing four acquisition strategies: Exploris 480 DDA/DIA, Astral HR-DIA, and timsTOF Ultra DIA-PASEF.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Lung

DISEASE(S): Bronchopulmonary Dysplasia

SUBMITTER: Filipa Blasco Tavares Pereira Lopes

LAB HEAD: Mark Chance

PROVIDER: PXD057442 | Pride | 2026-04-02

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	F004996.dat	Other
	F004996.mgf	Mgf
	F004996.mzid.gz	Mzid
	F004997.dat	Other
	F004997.mgf	Mgf

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Publications

What Does Next-Generation Mass Spectrometry Offer for Proteomics? A Comprehensive Platform Comparison.

Blasco Tavares Pereira Lopes Filipa F Schlatzer Daniela D Sudhadevi Tara T Harijith Anantha A Ayati Marzieh M Koyutürk Mehmet M Chance Mark R MR

Journal of proteome research 20260325 4

Next-generation mass spectrometry platforms (Orbitrap Astral, timsTOF Ultra) are reshaping proteomics by enhancing analytical depth and sensitivity. We compared these platforms against Orbitrap Exploris 480 using neonatal mouse lung tissues from a bronchopulmonary dysplasia model (<i>n</i> = 12), employing four acquisition strategies: Exploris 480 DDA/DIA, Astral HR-DIA, and timsTOF Ultra DIA-PASEF. All platforms identified ∼4000 proteins in common, with 98% proteome coverage of data-dependent a ...[more]

PMID: 41882496

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