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Unravelling Bovine Preadipocyte Differentiation and their Three-Dimensional Cultivation for Cellular Agriculture

ABSTRACT: Isolation and characterisation of Bovine preadipocytes harvested from adipoce tissue. The cell type and source was confirmed usinf RNA-seq, and the cell type was further confirmed through proteomics.

INSTRUMENT(S):

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Cell Culture, Preadipocyte

SUBMITTER: Apeksha Goswami

LAB HEAD: Prof. Johannes le Coutre

PROVIDER: PXD057468 | Pride | 2025-12-01

REPOSITORIES: Pride

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			Action	DRS
	240105_P33423_S0023451_SVC2-1ug_HFX_02.raw	Raw
	240105_P33423_S0023452_SVC3-1ug_HFX_03.raw	Raw
	240105_P33423_S0023453_SVC-I-1-1ug_HFX_04.raw	Raw
	240105_P33423_S0023455_SVC-I-3-1ug_HFX_06.raw	Raw
	240105_P33423_S0024472_SVC1B-1ug_HFX_01.raw	Raw

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Publications

Unravelling bovine preadipocyte differentiation and their three-dimensional cultivation for cellular agriculture.

Goswami Apeksha Bharatgiri AB Luu Laurence Don Wai LDW Biazik Joanna M JM Rybchyn Mark S MS le Coutre Johannes J

NPJ science of food 20251125 1

Cultured meat is an attractive concept to address environmental and ethical issues of livestock farming. Replication of conventional meat requires fat tissue, for flavour, texture, and mouthfeel. However, cultured meat production is limited by the lack of bovine adipogenic cells that are both proliferative and differentiable. Here, we demonstrate the isolation and characterisation of spontaneously immortalised bovine preadipocytes proliferating over 100 days and 21 passages, showing adipogenic d ...[more]

PMID: 41290777

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