Discovery of tomato UDP-glucosyltransferases involved in bioactive jasmonate homeostasis using limited proteolysis-coupled mass spectrometry

ABSTRACT: Jasmonic acid (JA) is the precursor of the plant hormone, jasmonoyl-isoleucine, with roles in defences and development. Molecular signalling pathways of JA are well-understood, but it is still not fully understood how JA is metabolised. We used limited proteolysis-coupled mass spectrometry to search for jasmonic acid (JA) interacting proteins in tomato with the aim of finding missing components of JA metabolism. We identified several UDP-glycosyltransferases (UGTs) that might be involved in direct glycosylation of JA, a metabolic step that is catalyzed by yet unknown enzymes. Two of the candidates from our dataset were confirmed to glycosylate JA in vitro to create JA-glucosyl ester (JA-Glc). JA-Glc was shown to have less biological activity than JA, suggesting that the discovered UGTs may be involved in inactivating JA for storage or catabolism.

INSTRUMENT(S):

ORGANISM(S): Solanum Lycopersicum

TISSUE(S): Hairy Root Culture

SUBMITTER: Kresimir Sola  

LAB HEAD: Alain Goossens

PROVIDER: PXD057493 | Pride | 2026-08-27

REPOSITORIES: Pride