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Human,scalp,dermal papilla cells,LC-MS/MS

ABSTRACT: Three individual patient-originated (Scalp trauma; ages 37, 46 and 57) DPCs were used for the studies. Dermal papilla cells, derived from the frontal scalp of 3 women, were treated with normal medium (Ctrl group) and medium containing 10-7 M corticotropin-releasing hormone (CRH group) for 72 h. Proteins were extracted and digested with trypsin for 4D label-free quantitative proteomics (Shanghai Applied Protein Technology Co., Ltd.).

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hepatocyte, Cell Culture

SUBMITTER: Wemzi Liang

LAB HEAD: Wenzi Liang

PROVIDER: PXD057745 | Pride | 2025-05-07

REPOSITORIES: Pride

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Publications

Corticotropin-releasing hormone inhibits autophagy by suppressing PTEN to promote apoptosis in dermal papilla cells.

Liang Wenzi W Chen Xiuwen X Ni Na N Zhuang Chutong C Yu Zhiying Z Xu Ziqing Z Li Yingshi Y Lin Changmin C Huang Keng K

Annals of medicine 20250412 1

<h4>Background</h4>Stress-related hair loss is on the rise, largely due to escalating levels of stress-related corticotropin-releasing hormone (CRH) through poorly defined mechanisms. CRH-mediated activation of corticotropin-releasing hormone receptors (CRHRs) on dermal papilla cells (DPCs) is a likely cause of stress-related hair loss. The aim of the study is to elucidate the key mechanisms of alopecia caused by CRH and provide potential new targets for the treatment of stress-related hair loss ...[more]

PMID: 40219757

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Vascular EC-derived factors promote DPC functions.

Project description:To explore the underlying molecular mechanism of crosstalk between endothelial cells (ECs) and dermal papilla cells (DPCs), we used RNA sequencing to investigate the transcriptional changes in the human follicle dermal papilla cells (HFDPC) culture induced by conditioned medium (CM) treatment from human umbilical vein endothelial cell (HUVEC) (EC-CM).

2024-11-26 | GSE282648 | GEO

Isolated of dermal papilla cells from follicular unit extraction

Project description:Hair follicles were originally obtained in the form of intact hair follicles from human scalp during microscopic hair transplant procedures of Follicular Unit Extraction (FUE). Dermal papilla cells were freshly isolated or traditionally isolated and cultured in basic medium supplied with 10% FBS. Freshly-isolated DPCs and cultured DPCs were sequenced using third generations of sequencing.

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The homing of exogenous hair follicle mesenchymal stem cells into hair follicle niches

Project description:In our study, we found that low-passage dermal papilla cells (DPCs) and dermal sheath cells (DSCs) has higher homing rate than that of high-passage DPCs and DSCs. To uncover the molecular mechanisms underlying the difference of homing rate between low-passage DPCs/DSCs and high-passage DPCs/DSCs, we perform transcriptome analysis of mRNA expression profiles of low-passage and high-passage DPCs and DSCs.

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AR/miR-221/IGF-1 pathway mediates the pathogenesis of androgenetic alopecia

Project description:Our data showed that miR-221 had a profound effect on the biological function of dermal papilla cells (DPCs) and dermal sheath cells (DSCs) of hair follicle. Overexpression of miR-221 affected the MAPK signaling in DPCs, and PI3K/AKT signaling in DSCs.

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Detection of the expression profiles of mRNA and miRNA between dermal papilla cells and hair matrix cells of yak hair follicle [RNA-seq]

Project description:Dermal papilla cells (DPCs) and epidermal hair matrix cells (HMCs) located in hair bulb are the key cell types during the hair follicles (HFs) development. To explore the mRNA and miRNA expression of DPCs and HMCs of yak hair follicle, illustrating the mocular basis of the interaction and cellular communication between DPCs and HMCs during the hair follicle development, the DPCs and HMCs of yak were isolated and cultured, RNA-seq was used to identify the differentially expressed mRNAs and miRNAs between DPCs and HMCs.

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Detection of the expression profiles of mRNA and miRNA between dermal papilla cells and hair matrix cells of yak hair follicle [miRNA-seq]

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Introduction of the TERT and BMI1 Genes into Murine Dermal Papilla Cells Ameliorates Hair Inductive Activity

Project description:Dermal papilla cells (DPCs) are located at the bottom of hair follicles and play important roles on hair induction by interacting with epidermal cells. DPCs are promising cell sources for hair regeneration therapy for alopecia patients. However, freshly isolated DPCs rapidly lose their hair inductive activity and proliferative potential under culture conditions. We examined the effects of telomere reverse transcriptase (TERT) and B-cell-specific Moloney murine leukemia virus insertion region 1 (BMI1) on extending the life span of murine DPCs as well as on hair inductive activity.TERT and BMI1 genes were introduced into murine DPCs using lentivirus vectors. Hair inductive activity of transfected DPCs (t-DPCs) was determined by in vivo chamber assay in nude mice. Transcripts between intact dermal papillae (DPs) and cultured DPCs were compared by microarray analysis.

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Gene expression profiling of total RNA isolated from hiPSC-derived DPPCs, hiPSC-derived NCSCs, cultured hDPC spheres, cultured hDPC

Project description:During development, dermal papilla precursor cells (DPPCs) initiate embryonic hair follicle (HF) formation with epidermal placode cells. Obtaining DPPCs with trichogenic ability is critical for human HF regeneration because dermal papilla cells (DPCs) rapidly loseDuring development, dermal papilla precursor cells (DPPCs) initiate embryonic hair follicle (HF) formation with epidermal placode cells. Obtaining DPPCs with trichogenic ability is critical for human HF regeneration because dermal papilla cells (DPCs) rapidly lose their trichogenic ability in culture. Here, we generated trichogenic DPPCs from human induced pluripotent stem cells (iPSCs) via neural crest stem cells (NCSCs), based on the developmental evidence at the hair placode stage. SDC1+CD133− cells showed signature DP gene expression, spontaneous sphere formation and represented intermediate population in the differentiation way from NCSCs to DPCs. hiPSC-derived DPPCs generated HF equivalents in vitro and reconstituted de novo human HFs in vivo combined with hiPSC-derived epithelial stem cells. Remarkably, trichogenic ability of DPPC was only proven in the specific time window, providing insights into the loss of trichogenicity in cultured DPC. Thus, this study provides an in vitro model for studying DPC development and biology.

2023-06-25 | GSE100793 | GEO

E. piscicida secretion effectors identification by LC-MS

Project description:The secretion protein of the important virulence factor T6SS has not been fully identified yet. In order to identify the novel T6SS secreted protein of important fish pathogens, we chose the quantitative proteomics method of 4D label free.

2024-12-28 | PXD050280 | Pride

Human dermal papilla cell culture medium iTRAQ

Project description:Alopecia is an exceedingly prevalent problem and lacks effective therapy. Recently, research has focused on early-passage dermal papilla cells (DPCs), which have hair inducing activity both in vivo and in vitro. Our previous study indicated that factors secreted from early-passage DPCs contribute to hair follicle (HF) regeneration. To identify which factors are responsible for HF regeneration and why late-passage DPCs lose this potential，we collected 48-h-culture medium (CM) from both of passage 3 and 9 DPCs and subcutaneously injected the DPC-CM into NU/NU mice. Passage 3 DPC-CM induced HF regeneration, based on the emergence of a white hair coat, but passage 9 DPC-CM not. In order to identify the key factors responsible for hair inductive capacity, CM from passage 3 and 9 DPCs was analyzed by iTRAQ-based quantitative proteomic technology. We identified 1360 proteins, of which 213 proteins were differentially expressed between CM from early-passage vs. late-passage DPCs, including SDF1, MMP3, biglycan and LTBP1.Further analysis indicated that the differentially-expressed proteins regulated the Wnt, TGF-β and BMP signaling pathways, which directly and indirectly participate in HF morphogenesis and regeneration. Subsequently, we selected 19 proteins for further verification by multiple reaction monitoring (MRM) between the two types of CM. These results would be used to identify the key factors for inducing HF regeneration and reveal the molecular mechanisms of losing inductive ability of DPCs. Furthermore, it is possible to explore some drugs for alopecia in the clinic according to this secretome date analysis.

2018-10-26 | PXD004201 | Pride

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