Dataset Information

The proteomic and peptidomic response of wheat (Triticum aestivum L.) to drought stress

ABSTRACT: Drought negatively impacts the morphological, physiological, and molecular characteristics of plants. Here we performed quantitative proteomic and peptidomic analysis of spring wheat (Triticum aestivum L.). leaves and roots under drought stress conditions. Using isobaric tags for relative and absolute quantitation (iTRAQ), we identified 497 and 157 differentially expressed (DE) proteins in leaves and roots, respectively. The upregulated DE proteins in leaves were primarily involved in stress responses, such as oxidative stress and heat response, while those in roots were associated with metabolic processes like UDP-glucose metabolism and glutamine biosynthesis. Using peptidomic approaches, we identified 2294 endogenous extracellular peptides, including members of 16 small secreted peptides (SSPs) families, in the root secretome. In the stress-induced secretomes, we identified endogenous peptides, derived from proteins involved in cell wall catabolism and detoxification and involved in intercellular signaling and stress response. Identification of proteins and secretome peptides regulating the drought stress response helps to understand adaptation mechanisms and develop new agricultural technologies to increase productivity.

INSTRUMENT(S):

ORGANISM(S): Triticum Aestivum (wheat)

TISSUE(S): Culture Fluid, Plant Cell, Root, Leaf

SUBMITTER: Igor Fesenko

LAB HEAD: Regina Azarkina

PROVIDER: PXD057751 | Pride | 2025-08-04

REPOSITORIES: Pride

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Publications

The Proteomic and Peptidomic Response of Wheat (<i>Triticum aestivum</i> L.) to Drought Stress.

Azarkina Regina R Makeeva Arina A Mamaeva Anna A Kovalchuk Sergey S Ganaeva Daria D Tikhonovich Igor I Fesenko Igor I

Plants (Basel, Switzerland) 20250714 14

Drought conditions impact plants at the morphological, physiological, and molecular levels. Plant tolerance to drought conditions is frequently associated with maintaining proteome stability, highlighting the significance of proteomic analysis in understanding the mechanisms underlying plant resilience. Here, we performed proteomic and peptidomic analysis of spring wheat (<i>Triticum aestivum</i> L.) under drought stress conditions. Using isobaric tags for relative and absolute quantitation (iTR ...[more]

PMID: 40733406

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Project description:Drought stress response is a complex trait regulated at multiple levels. In the past few years, molecular and genomic studies have shown that several drought responsive genes (DRGs) with various functions are induced by drought stresses, and that various transcription factors (TFs) are involved in the regulation of stress-inducible genes. In addition to those DRGs mentioned above, microRNAs (miRNAs) are important regulators of gene expression at the posttranscriptional level by repressing mRNA expression. There is a complex interplay between transcriptional and post-transcriptional regulation of drought response that has not been extensively characterized in tobacco. In order to fully understand DRGs (including TFs) and different roles of miRNAs involved in the stress response, we sequenced and analysed three Digital Gene Expression (DGE) libraries in roots from drought treated tobacco plants, and four small RNA populations in roots, stems and leaves from control or drought treated tobacco plants. We identified 276 candidate DRGs in tobacco with sequence similarities to 64 known DRGs from model plants and crops and about 40% were TFs including WRKY, NAC, ERF and bZIP families. Furthermore, Out of these tobacco DRGs, 54differentially expressed DRGs included 21 TFs, which belonged to 24 TFs families such as NAC (6), MYB (4), ERF (10) and bZIP (1). Additionally, we confirmed expression of 39 known miRNA families (122 members) and five conserved miRNA families, which showed differential regulation under drought stress. Targets of miRNAs were further surveyed based on a recently published study, in which ten targets were DRGs. Finally, an integrated gene regulatory network has been proposed for the molecular mechanisms of the response of tobacco roots to drought stress using differentially expressed DRGs, the changed expression proﬁles of miRNAs and their target transcripts as a basis base on previous studies. In general, our data provide valuable information for future studies of the molecular mechanisms underlying tobacco roots in response to drought resistance in tobacco and other plants. Three tobacco (Nicotiana tabacum L.) roots tag-based DGE libraries treated at three time points (0, 6 and 48 h) with 20% PEG6000 were generated using Illumina 1G technology. The samples for four small RNA libraries was used based on the result of physiological index measurement as follows: equal quantities (10 ug) of total RNA isolated from tobacco roots treated with two time points (6 and 48 h) were mixed together to construct the drought -treated small RNA library (Root-treat), and total RNA prepared from the control roots sample was used to construct the control small RNA library (Root-ck). In addition, we also constructed another two libraries (stem and leaf) from leaves and stems of control plants, respectively. These libraries were constructed using the Small RNA Sample Prep Kit (Illumina, San Diego, CA) and then sent for Solexa sequencing.

Project description:In the field, abiotic stresses are rarely applied individually. Crops are often subjected to a combination of stresses. To date, no study has been performed on the proteomic investigation of the response of common wheat to a combination of drought and cold stresses. In this study, wheat seedlings exposed to drought-cold stress for 24 h showed inhibited growth, increased lipid peroxidation, relative electrolyte leakage, and soluble sugar contents. To determine the wheat protein response to drought-cold stress, iTRAQ-based quantitative proteomic and liquid chromatography tandem mass spectrometry (LC-MS/MS) methods were employed to determine the proteomic profiles of the roots and leaves of wheat seedlings exposed to drought-cold stress conditions. We identified 250 and 258 proteins with significantly altered abundance in the roots and leaves, respectively. These proteins were classified into several main groups, as follows: protein metabolism, stress/defense, carbohydrate metabolism, lipid metabolism, transcription-related processes, energy production, cell-wall and cytoskeleton metabolism, membrane and transportation, signal transduction, other metabolic processes, and unknown biological processes. Nine proteins were simultaneously presented in both roots and leaves exposed to drought-cold stress, and the majority of proteins identified differed from one another and displayed differently altered abundance. These findings uncovered organ-specific differences in adaptation to drought-cold stress. Exogenous abscisic acid (ABA) application conferred the plant with protection against drought-cold stress and significantly increased catalase and peroxidase enzyme activities, as well as the transcription of glutathione S-transferase and other 11 sample genes in the roots or leaves, respectively. These results suggested that ABA is a potentially vital factor that contributes to the drought-cold signaling pathway and a promising target for growth recovery. Furthermore, VIGS (virus-induced gene silencing)-treated plants generated for three candidate protein genes TaGRP2, CDCP and WCOR410c were subjected to drought-cold limitation, they showed more serious droop and wilt, increased rate of relative electrolyte leakage, and reduced relative water content (RWC) compared to viral control plants. These results may indicate that TaGRP2, CDCP and WCOR410c play important roles in conferring drought-cold tolerance in wheat. These findings can provide useful insights into the molecular mechanisms of drought-cold responses in higher plants.

Dataset Information

The proteomic and peptidomic response of wheat (Triticum aestivum L.) to drought stress

Dataset's files

Publications

The Proteomic and Peptidomic Response of Wheat (<i>Triticum aestivum</i> L.) to Drought Stress.

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Dataset Information

The proteomic and peptidomic response of wheat (Triticum aestivum L.) to drought stress

Dataset's files

Publications

The Proteomic and Peptidomic Response of Wheat (&lt;i&gt;Triticum aestivum&lt;/i&gt; L.) to Drought Stress.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

The Proteomic and Peptidomic Response of Wheat (<i>Triticum aestivum</i> L.) to Drought Stress.