Dataset Information

Formula Q attenuates letrozole-induced polycystic ovary syndrome through modulating multiple pathways in rat

ABSTRACT: In this research, we investigated the molecular mechanisms and signaling pathways of Formula Q attenuating PCOS using a Tandem Mass Tag (TMT) proteomics approach, bioinformatics analyses and molecular biological technology. This study aimed to comprehensively characterize the multiple molecular mechanisms of PCOS progress improved by Formula Q and to explore the potential target and pathway covering multiple pathogeneses of PCOS.

INSTRUMENT(S):

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Ovary

DISEASE(S): Polycystic Ovary Syndrome

SUBMITTER: WU JingYi

LAB HEAD: Yao Xiaomin

PROVIDER: PXD057910 | Pride | 2026-05-25

REPOSITORIES: Pride

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Chinese herbal Formula Q attenuates letrozole-induced polycystic ovary syndrome through modulating multiple metabolic processes in female adult rats.

Yin Yihong Y Wu Jingyi J Shen Ying Y Lin Qidi Q Zhang Xiaofang X Jia Shu S Shao Jingping J Yao Xiaomin X Ye Liqun L

In silico pharmacology 20260516 2

Polycystic ovary syndrome (PCOS) is driven by multiple factors, but detailed mechanisms in the setting of PCOS remains unclear. Our study is to comprehensively clarify the potentially molecular mechanisms of effect Formulae Q (Traditional Chinese Medicine) on PCOS. A model of PCOS-liked rats induced by letrozole (1 mg/kg/d) for 21days was performed. Formulae Q (20 g/kg/d) was orally gavaged twice daily to rats as a pretreatment, and metformin (100 mg/kg/d) was orally gavaged daily as a positive ...[more]

PMID: 42153178

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Project description:BACKGROUND: Polycystic ovarian syndrome (PCOS) is a common endocrine disorder characterized by hyperandrogenism, ovarian dysfunction and polycystic ovarian morphology. Gut microbiota dysbiosis and metabolite are associated with PCOS clinical parameters. Yulin Tong Bu formula (YLTB), a traditional Chinese medicine formula, has been recently indicated to be capable of ameliorating polycystic ovary symptoms and correcting abnormal glucose metabolism. However, the therapeutic mechanism of YLTB on PCOS has not been fully elucidated.METHODS: A pseudo sterile mouse model was established during this 4-day acclimatization phase by giving the animals an antibiotic cocktail to remove the gut microbiota. Here, the therapeutic effects of YLTB on PCOS were investigated using dehydroepiandrosterone plus high-fat diet induced PCOS mice model. Female prepuberal mice were randomly divided into 3 groups; namely, the control group, PCOS group and YLTB (38.68 g/kg/day) group. To test whether this effect is associated with the gut microbiota, we performed 16S rRNA sequencing studies to analyze the fecal microbiota of mice. The relationships among metabolites, gut microbiota and PCOS phenotypes were further explored by using Spearman correlation analysis. Then, the effect of metabolite ferulic acid was then validated in PCOS mice.RESULTS: Our results showed that YLTB treatment ameliorated PCOS features (ovarian dysfunction, delayed glucose clearance, decreased insulin sensitivity, deregulation of glucolipid metabolism and hormones, etc) and significantly attenuated PCOS gut microbiota dysbiosis. Spearman correlation analysis showed that metabolites such as ferulic acid and folic acid are negatively correlated with PCOS clinical parameters. The effect of ferulic acid was similar to that of YLTB. In addition, the bacterial species such as Bacteroides dorei and Bacteroides fragilis were found to be positively related to PCOS clinical parameters, using the association study analysis.CONCLUSION: These results suggest that YLTB treatment systematically regulates the interaction between the gut microbiota and the associated metabolites to ameliorate PCOS, providing a solid theoretical basis for further validation of YLTB effect on human PCOS trials.

			Action	DRS
	Set_of_12_files.pdResult	Other
	WJY_TMT_1.raw	Raw
	WJY_TMT_10.raw	Raw
	WJY_TMT_2.raw	Raw
	WJY_TMT_3.raw	Raw