Dataset Information

Proteomic Profile at Revision Arthroplasty for Periprosthetic Joint Infection Correlates with Disease Stage and Surgical Outcome

ABSTRACT: Periprosthetic joint infection (PJI) is both the most common and difficult to treat form of arthroplasty failure, occurring in ~2% of primary total knee and hip arthroplasties. For patient and surgeon alike, treating PJI with DAIR surgery (debridement, antibiotics, and implant retention) is preferable to implant resection surgery due to medical costs, patient morbidity, and required skill, however, DAIR success rates are variable. Therefore, an accurate diagnostic to identify candidates who will undergo DAIR successfully would be clinically helpful. To achieve this, advanced proteomic analysis of sonicate fluid from 95 subjects with PJI was performed using proximity extension assay (PEA), with 77 samples additionally undergoing liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis. A group of 120 proteins was identified that successfully separates DAIR samples into success or failure categories and predicts DAIR surgery outcome post-hoc. Additionally, the proteomic signature associated with short symptom duration and long symptom duration PJI was defined and used to categorize samples into 2 putative stages of infection. The investigation of 7,261 unique human proteins was employed here to explore the physiology of PJI and reveals a proteomic signature for DAIR failure as well as insight into PJI protein profiles based on patient reported symptom duration.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Bodily Fluid

DISEASE(S): Joint Inflammation

SUBMITTER: Akhilesh Pandey

LAB HEAD: Akhilesh Pandey

PROVIDER: PXD058084 | Pride | 2025-08-26

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	SonicateFluid_ddaPASEF_Rawfiles.zip	Other
	combined_protein.tsv	Tabular

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Project description:Sacrocolpopexy has been dubbed the âgold standardâ repair for apical pelvic organ prolapse (POP). This study sought to determine a genetic cause for sacrocolpopexy failure by comparing genotypes from 10 women who suffered from early POP reoccurance after sacrocolpopexy surgery, versus 40 randomly selected women with long term success after the same procedure. We objectively defined early overt failure after robotic-assisted laparoscopic sacrocolpopexy as having a pelvic organ prolapse quantification system examination (POP-Q) of stage III or IV occurring in more than one compartment within six months after surgery. All medical records identified during this process were then reviewed by a panel of urogynecology attendings and fellows to select patients who were truly clinical outliers. By this method we identified 10 patients (cases) who experienced early overt surgical failure. We also randomly selected 40 controls from our research database which includes greater than 500 patients who underwent robotic-assisted laparoscopic sacrocolpopexy during the same time period and had been objectively and subjectively assessed for â¥ 12 months with surgical success at â¥ 12 months that did not undergo prolapse re-operation or re-treatment. Demographics and peri-operative details were compared between cases and controls. Exclusion criteria for controls included use of other graft material besides polypropylene mesh, prior surgery for prolapse involving graft material, and conversion to laparotomy. DNA from the 10 cases and 40 controls was isolated from buccal swabs and genotyped on a single nucleotide polymorphism (SNP) array that contains 250,000 markers (NspI 250K SNP array, Affymetrix, Santa Clara, CA). All women in this study identified as Caucasian. All subjects provided written informed consent to study participation and data release. This was a case-control study approved by the Institutional Review Board at the Atlantic Health System in Morristown New Jersey (R11-10-004). This case-control study compared single genotypes of 10 cases to 40 controls. All subjects were identified as Caucasian. Cases were women who experienced early overt POP recurrence after robotic sacrocolpopexy, and controls were randomly selected women with long term success after the same procedure.

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Proteomic Profile at Revision Arthroplasty for Periprosthetic Joint Infection Correlates with Disease Stage and Surgical Outcome

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