Dataset Information

Intestinal inflammation induces remodelling in cerebrospinal fluid protein composition

ABSTRACT: Inflammatory bowel disease has been reported to trigger a variety of extra-intestinal manifestations in other organs, including the central nervous system (CNS). However, the direct impact of peripheral inflammation on the CNS is largely unknown. Cerebrospinal fluid (CSF) is a clear, colourless body fluid present in the brain and spinal cord. It performs vital functions since it protects the brain from injury and helps in maintaining a stable chemical environment for the central nervous system, acting as waste removal. Because CSF is in direct contact with the extracellular space of the brain, it provides insight into the status of the CNS. The analysis of changes in CSF proteome of murine models could help researchers in understanding the pathophysiology of several neurological diseases; however, the small volume of CSF in mice has thus far limited individual mouse proteome characterization. Moreover, the wide dynamic range of protein concentrations in CSF requires specific sample preparation for label-free proteomics. In order to address the impact of inflammatory bowel disease on CNS, we used a mouse model of intestinal painful inflammation, i.e. mice treated with 2.5 % dextran sodium sulphate (DSS) (w:v) in tap water ad libitum from day 0 until day 5, followed by DSS-free water in the next three days. By comparing the CSF protein content from 8 DSS-treated mice to that one derived from 7 control mice, we unveiled a pathogenic association between peripheral colonic inflammation and signals of early CNS alterations. Marked changes in cerebrospinal fluid protein composition were observed by untargeted proteomics, revealing enrichment in proteins related to inflammatory, immune response, complement, neuronal, and lipid metabolic pathways.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cerebrospinal Fluid

DISEASE(S): Irritable Bowel Syndrome

SUBMITTER: Annapaola Andolfo

LAB HEAD: Cristina Lanni

PROVIDER: PXD058129 | Pride | 2026-05-13

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

Items per page:

1 - 5 of 46

Similar Datasets

Project description:Background. Flow cytometry (FCM) identifies leukemic blasts in cerebrospinal fluid (CSF) in approximately 30% of children with acute lymphoblastic leukemia (ALL), whereas conventional cytospin preparations frequently miss such involvement. <br>Procedure. To explore biological properties of leukemic cells that migrate into central nervous system (CNS), we compared gene expression profiles of leukemic cells from 26 patients with CNS involvement and 138 patients without CNS leukemia as determined by FCM and/or cytospin preparations. Results were validated by real-time quantitative PCR (qPCR). <br>Results. We found the cysteine endopeptidase legumain (LGMN) that plays a role in hydrolysis of proteins and small molecules to be significantly differentially expressed between FCM+ and FCM- B-cell precursor (BCP) ALL patients. When LGMN expression was validated by qPCR it stayed differentially expressed between FCM+ and FCM- BCP-ALL patients (P=0.006), and also between FCM+ and cytospin-negative BCP-ALL patients (P=0.01). In multivariate regression analysis including sex, age and WBC, LGMN expression levels above population median were associated with an odds ratio of 9.1 for CNS involvement as determined by FCM compared with expression less than the median (95% CI, 1.26 to 65.50; P=0.03). Conclusions. The use of more sensitive methods for detecting and quantifying CNS involvement, such as FCM, may facilitate detection of markers of leukemia biology, such as LGMN, which could have therapeutical implications for future CNS-directed therapy.<br>FCM+: Patients had leukemic cells in the cerebrospinal fluid (CSF) detected by flow cytometry and no blood contamination. FCM-: Patients had no leukemic cells in the CSF detected by flow cytometry with or without blood contamination.<br>CNS1: No leukemic cells in CSF detected by cytospin examination with or without blood contamination and no other clinical signs of central nervous system (CNS) leukaemia. CNS2: CSF leukocyte count <5 x106/ÂµL with blasts on cytospin examination and no blood contamination. CNS3: CSF leukocyte count >5 x106/ÂµL with blasts on cytospin examination and no blood contamination, and/or cranial nerve palsy, and/or CNS leukaemia by neuroimaging.

			Action	DRS
	CL_CTR10_a.mgf	Mgf
	CL_CTR10_a.raw	Raw
	CL_CTR3_a.mgf	Mgf
	CL_CTR3_a.raw	Raw
	CL_CTR5_a.mgf	Mgf

Dataset Information

Intestinal inflammation induces remodelling in cerebrospinal fluid protein composition

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets