Dataset Information

SIMPLEX enriches hydrophobic and lipidated proteins in membrane proteomics experiments

ABSTRACT: Synaptosome (Syn) and synaptic junctions (SJ) are widely used in omics experiments to investigate protein content and networks of synapses. Synapses are a neuronal compartment wit a high lipid content. Liquid-liquid extraction (LLE), typically used in lipidomics, shows great potential for multiomics experiments, where data is generated from the same sample, due to its ability to integrate different molecule classes in one extraction. However, the influence of liquid-liquid extraction on protein and phospho-proteins analysis of membrane-enriched samples has not been investigated nor compared to one-phase extraction. In this study, we compared the suitability of SIMPLEX (Simultaneous Metabolite, Protein, Lipid Extraction)- an example of LLE- for mass-spectrometry based protein identification to a commonly used acetone protein precipitation.

INSTRUMENT(S):

ORGANISM(S): Rattus Rattus (black Rat)

TISSUE(S): Brain

SUBMITTER: Tingting Li

LAB HEAD: Robert Ahrends

PROVIDER: PXD058138 | Pride | 2025-09-08

REPOSITORIES: Pride

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SIMPLEX Enriches Hydrophobic and Lipidated Proteins in Membrane Proteomics Experiments.

Li Tingting T Wenger Alexander A Coman Cristina C Borutzki Corinna C Kreutz Michael R MR Ahrends Robert R

Proteomics 20250808 16

Synaptosomes (Syn) and synaptic junctions (SJ) are key neuronal compartments that have been widely characterized in omics studies to understand neurotransmitter- and signal transduction-related events. While synapses are lipid-rich, multiomics approaches integrating lipids and proteins remain largely underexplored. Liquid-liquid extraction (LLE), commonly used in lipidomics, offers significant potential for multiomics analyses by enabling the extraction of diverse molecular classes from a single ...[more]

PMID: 40776855

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