Proteomics

Dataset Information

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Proteomic analysis of WT macrophages that have phagocytosed SILAC-bacteria

ABSTRACT: Macrophage are phagocytic cells that reprogram their cellular metabolism depending on the nature of innate immune receptors engaged upon microbial encounter. Yet, whether engulfed microbes constitute a source of nutrients for macrophages and how this contribute at establishing a specific metabolic reprogramming is poorly defined. Our study aims at understanding how microbial-derived amino acids feed macrophage metabolism by tracing labelled microbial amino acid in macrophage proteins. Here we analyzed the incorporation of labelled amino acids derived from dead bacteria into macrophage proteins at different time points.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Escherichia Coli Mus Musculus (mouse)

SUBMITTER: Dupuy Jean-William  

LAB HEAD: Johan Garaude

PROVIDER: PXD058142 | Pride | 2025-04-18

REPOSITORIES: Pride

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Dataset's files

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Action DRS
Link_between_files.xlsx Xlsx
RP_UP000000589_Mus_musculus_2024_03_21.fasta Fasta
UP_UP000000625_Ecoli_K12_2024_03_21.fasta Fasta
checksum.txt Txt
jg181115_01.msf Msf
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Publications

Macrophages specialize in phagocytosis, a cellular process that eliminates extracellular matter, including microorganisms, through internalization and degradation<sup>1,2</sup>. Despite the critical role of phagocytosis during bacterial infection, the fate of phagocytosed microbial cargo and its impact on the host cell are poorly understood. In this study, we show that ingested bacteria constitute an alternative nutrient source that skews immunometabolic host responses. By tracing stable isotope  ...[more]

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