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HDX-MS Studies of Dengue Virus Serotype 2 NS5 (DENV2 NS5) and Stem Loop A (SLA) Interactions

ABSTRACT: The project employs HDX-MS to study the conformational dynamics of DENV2 NS5 in its apo form and in complex with the viral promoter RNA, Stem Loop A (SLA). The conformational dynamics of the RdRp domain of DENV2 NS5 (NS5-RdRp) in its apo form and in complex with SLA was also studied.

INSTRUMENT(S):

ORGANISM(S): Escherichia Coli

SUBMITTER: Juliet Obi

LAB HEAD: Daniel J. Deredge

PROVIDER: PXD058297 | Pride | 2025-05-07

REPOSITORIES: Pride

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			Action	DRS
	DENV2_NS5.zip	Other
	DENV2_NS5_Seq_1_reducedASMflow_05_11_2023_IA_workflow.xml	Xml
	DENV2_NS5_Seq_2_reducedASMflow_05_11_2023_IA_workflow.xml	Xml
	DENV2_NS5_Seq_3_reducedASMflow_05_11_2023_IA_workflow.xml	Xml
	DENV2_RdRp.zip	Other

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Publications

Structural dynamics of the dengue virus non-structural 5 (NS5) interactions with promoter stem-loop A (SLA).

Obi Juliet O JO Kihn Kyle C KC McQueen Linfah L Fields James K JK Snyder Greg A GA Deredge Daniel J DJ

Npj viruses 20250416 1

The dengue virus (DENV) NS5 protein, essential for viral RNA synthesis, is an attractive antiviral drug target. DENV NS5 interacts with the stem-loop A (SLA) promoter at the 5'-untranslated region of the viral genome to initiate negative-strand synthesis. However, the conformational dynamics of this interaction remains unclear. Our study explores the structural dynamics of DENV serotype 2 NS5 (DENV2 NS5) in complex with SLA, employing surface plasmon resonance (SPR), hydrogen-deuterium exchange ...[more]

PMID: 40295851

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Structure and dynamics of GAD65 and its interaction with the autoimmune polyendocrine syndrome type 2-associated autoantibody b96.11.

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Structural dynamics in the evolution of SARS-CoV-2 spike glycoprotein: HDX-MS analysis of the spike of alpha, beta, delta and omicron variants - in apo state and in complex with the ACE2 receptor

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