Dataset Information

Ocular Inoculation of Toad Venom: Toxic Cataract and Proteomic Profiling

ABSTRACT: Purpose: To report a singular case of cataract caused by toad venom inoculation and to scrutinize the pathological mechanisms through proteomic sequencing of the lens specimen. Methods: A young Chinese male presented with progressively deteriorating vision in his right eye subsequent to a history of toad venom inoculation. He was diagnosed with a toxic cataract, and underwent phacoemulsification cataract surgery. Anterior capsule, nucleus, and cortex specimens from the patient (designated as PT_CAP, PT_PHACO, and PT_CTX, respectively) and age-related cataract controls (C_CAP, C_PHACO, and C_CTX, respectively) were collected and subjected to 4D label-free quantitative proteomics. Results: A multitude of differentially expressed proteins (DEPs) were identified in the patient's lens compared to those in the controls. Specifically, a total of 204 DEPs were identified in PT_CAP compared to C_CAP, with MYH6, MYL2, MYL3, STAT1, and ANK1 among the foremost regulated DEPs. The DEPs of PT_CAP were principally affiliated with functions including "transportation of small molecules", "regulation of metal ion transport", and "import into cell". A sum of 109 DEPs were delineated in PT_CTX compared to C_CTX, with TPM1 among the top-10 downregulated DEPs. Ninety-five DEPs were pinpointed in PT_PHACO compared to C_PHACO, with hexokinase among the top 10 downregulated DEPs. These proteins were ascertained to be linked with Na+/K+-ATPase activity. Conclusion: This study introduced the first documented case of toxic cataract caused by toad venom inoculation. Proteomic sequencing indicated a correlation between cataract and alterations in Na+/K+-ATPase activity, providing insights for the clinical management of ocular toad venom inoculation in subsequent cases.

INSTRUMENT(S): timsTOF Pro 2

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Lens

SUBMITTER: Danni Lyu

LAB HEAD: Wen Xu

PROVIDER: PXD058328 | Pride | 2025-05-13

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	FPEP210059639-1A_Slot1-31_1_298.d.zip	Other
	FPEP210059640-1A_Slot1-32_1_299.d.zip	Other
	FPEP210059641-1A_Slot1-33_1_300.d.zip	Other
	FPEP210059644-1A_Slot1-34_1_301.d.zip	Other
	FPEP210059645-1A_Slot1-35_1_302.d.zip	Other

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Publications

Ocular inoculation of toad venom: toxic cataract and proteomic profiling.

Lyu Danni D Ni Shuang S Xu Jia J Zhu Sha S Xu Jing-Wei JW Feng Yixuan Y Shi Ce C Xu Wen W

Frontiers in medicine 20250114

<h4>Purpose</h4>To report a singular case of cataract caused by toad venom inoculation and to scrutinize the pathological mechanisms through proteomic sequencing of the lens specimen.<h4>Methods</h4>A young Chinese male presented with progressively deteriorating vision in his right eye subsequent to a history of toad venom inoculation. He was diagnosed with a toxic cataract, and underwent phacoemulsification cataract surgery. Anterior capsule, nucleus, and cortex specimens from the patient (desi ...[more]

PMID: 39876868

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