Dataset Information

CEPD/ROXY6-9 proteins activate N starvation response genes by interfering with the repressive activity of transcription factors TGA1 and TGA4

ABSTRACT: Fluctuations in the supply of soil nitrogen (N) cause profound alterations in the transcriptome of plants, allowing improved N acquisition, allocation and remobilization. In Arabidopsis thaliana, N starvation-induced CEPD (C-TERMINALLY ENCODED PEPTIDE DOWNSTREAM) proteins are required for transcriptional activation of genes involved in efficient nitrate uptake by roots. CEPDs belong to a plant-specific class of glutaredoxin-like proteins (called ROXYs in A. thaliana) that interact with TGACG-binding transcription factors (TGAs). Here we show that TGA1 and TGA4 act as molecular links between CEPDs/ROXY6-9 and target promoters in roots. In the absence of CEPDs/ROXYs 6-9, TGA1/4 recruit a strong repressive complex. In wild-type plants, this repressive complex is dysfunctional and transcriptional activation upon N starvation is most likely regulated by the increased ratio of CEPDs/ROXYs 6-9 over antagonistically acting ROXYs encoding the TOPLESS-interacting ALWL motif. TGA1/4 are required for transcriptional repression on full N supply and/or for transcriptional activation under N starvation, depending on the promoter and on the exact composition of the medium. Although CEPDs/ROXY6-9 resemble glutaredoxins with glutathione-dependent oxidoreductase activity, complementation analysis with ROXY9 variants encoding mutations in the active site excludes that ROXY9 functions by altering the redox state of target proteins.

INSTRUMENT(S):

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Root

SUBMITTER: Corinna Thurow

LAB HEAD: Christiane Gatz

PROVIDER: PXD058335 | Pride | 2025-05-07

REPOSITORIES: Pride

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Publications

Redox-inactive CC-type glutaredoxins interfere with TGA transcription factor-dependent repression of target promoters in roots.

Thurow Corinna C Pelizaeus Anja Maren AM Mrozek Pascal P Hoßbach Ben Moritz BM Budimir Jelena J Schmitt Kerstin K Valerius Oliver O Braus Gerhard G Gatz Christiane C

The Plant cell 20250301 3

Changes in nitrogen (N) availability in the soil trigger transcriptional responses in plants to optimize N acquisition, allocation, and remobilization. In roots of N-starved Arabidopsis (Arabidopsis thaliana) plants, transcriptional activation of genes encoding, for example, low-affinity nitrate transporters, depends on 4 related C-TERMINALLY ENCODED PEPTIDE DOWNSTREAM (CEPD) proteins, also known as ROXY6, ROXY7, ROXY8, and ROXY9. All 21 ROXYs found in A. thaliana interact with members of the TG ...[more]

PMID: 40053521

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