Dataset Information

GALNT11 Controlled O-glycans Modulate Ligand Interactions of the Endocytic Receptor LRP1

ABSTRACT: Low-density lipoprotein receptor (LDLR) and LDLR-related proteins (LRPs) are essential endocytic receptors and regulators of multiple physiological processes including protein reabsorption, cholesterol clearance, and neuronal protein trafficking. Previously, we identified O-GalNAc glycans within ligand-binding regions of LRP receptors, with functional implications for the uptake of LDL (via LDLR) and albumin (via LRP2). Notably, these O-glycans are specifically introduced by GALNT11, one of 20 GALNT isoenzymes. Here, we used cell models to investigate the role of GALNT11-mediated glycosylation in modulating ligand interactions of the widely expressed receptor LRP1. We find that while GALNT11-specific glycosylation does not alter LRP1-mediated uptake of ligands such as ApoE and RAP, it significantly affects the uptake of tau, amyloid beta and Hemopexin. We further show that GALNT11 O-glycans on LRP1 are selectively hyposialylated, and molecular dynamics simulations reveal that their sialylation can prevent glycan-ligand interactions.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cerebrospinal Fluid

SUBMITTER: Sergey Vakhrushev

LAB HEAD: Sergey Vakhrushev

PROVIDER: PXD058370 | Pride | 2026-03-02

REPOSITORIES: Pride

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			Action	DRS
	210821_Lumos_LC1200_nanoFlex_john_Biotin_TMT_50_HCD_37.raw	Raw
	210916_Lumos_LC1200_nanoFlex_John_TMT6_biotin_enriched_2h_HCD_37_Shifa2.raw	Raw
	220115_Lumos_LC1200_John_TMT4_biotin_enriched_CSF2_neutravidin_1.raw	Raw
	220115_Lumos_LC1200_John_TMT4_biotin_enriched_CSF2_neutravidin_2.raw	Raw
	220429_Lumos_Asli_E1E2_CSF.raw	Raw

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GALNT11 Controlled O-glycans Modulate Ligand Interactions of the Endocytic Receptor LRP1

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