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Proteomics of zebrafish early somites

ABSTRACT: The segmented pattern of the vertebral column and musculature in vertebrate embryos is established during somitogenesis. Here, using the model animal zebrafish, we employed Data-Independent Acquisition (DIA) to capture the proteomics change at the early stages of somite development.

INSTRUMENT(S):

ORGANISM(S): Danio Rerio (zebrafish) (brachydanio Rerio)

TISSUE(S): Embryo, Somite

SUBMITTER: Guangyu SUN

LAB HEAD: Guangyu Sun

PROVIDER: PXD058516 | Pride | 2025-10-13

REPOSITORIES: Pride

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			Action	DRS
	20230615_SGY_SG-A2.raw	Raw
	20230615_SGY_SG-A3.raw	Raw
	20230615_SGY_SG-A4.raw	Raw
	20230615_SGY_SG-C1.raw	Raw
	20230615_SGY_SG-C2.raw	Raw

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Publications

Actn4 links inactive Integrin α5 with actin in zebrafish somites.

Sun Guangyu G Holley Scott A SA

Molecular & cellular proteomics : MCP 20251008

Integrins are key plasma membrane proteins mediating cell-ECM adhesion and communication and rely on a conformational change for their activation and bidirectional signaling. However, there are few in vivo studies of integrin activation. Here, we identify Integrin α5 (Itgα5) associated proteins in the physiological setting of zebrafish somite morphogenesis. Using label-free mass spectrometry, we compared Itgα5-associated proteins in different integrin activation states. As expected, we found act ...[more]

PMID: 41072919

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