Dataset Information

DIA quantitative protein mass spectrometry of RBE cells knockout NAT10

ABSTRACT: To further explore the role of NAT10 in ICC, we constructed stable NAT10-knockdown RBE cells using shRNA and performed DIA quantitative proteomic analysis to explore its influence on downstream protein fuction.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cholangiocarcinoma Cell Line

DISEASE(S): Cholangiocarcinoma

SUBMITTER: Kainan Lin

LAB HEAD: Junjie Xu

PROVIDER: PXD058708 | Pride | 2026-05-25

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	Astral-2_24_FPEP240108520-1A.raw	Raw
	Astral-2_24_FPEP240108521-1A.raw	Raw
	Astral-2_24_FPEP240108522-1A.raw	Raw
	Astral-2_24_FPEP240108523-1A.raw	Raw
	Astral-2_24_FPEP240108524-1A.raw	Raw

Items per page:

1 - 5 of 8

Publications

NAT10/ac<sup>4</sup>C drives intrahepatic cholangiocarcinoma by suppressing transposable elements via chromatin remodeling.

Lu Yunkun Y Lin Kainan K Wang Qianqian Q Huang Xiang X Lin Lianyu L Dou Jie J Jiao Yajuan Y Wang Yali Y Xiao Peng P Chen Hongjun H Li Silin S Xue Yangtao Y He Shunmin S Xu Junjie J

Proceedings of the National Academy of Sciences of the United States of America 20260514 20

Intrahepatic cholangiocarcinoma (ICC) poses a significant clinical challenge due to its insidious onset, aggressive biological behavior, and propensity for early metastasis, contributing to a dismal 5-y survival rate of less than 10%. Despite advances in understanding the dynamic changes in gene regulatory networks and chromatin landscapes during tumorigenesis, the functional interplay between RNA epitranscriptomic modifications and nuclear events in ICC remains poorly elucidated. Here, we ident ...[more]

PMID: 42133812

Similar Datasets

Project description:Despite the demonstrated efficacy of immune checkpoint blockade therapies in various tumors, their efficacy in cervical cancer is limited by factors such as the need for positive PD-L1 expression and the development of drug resistance. A crucial pro-inflammatory cytokine, macrophage migration inhibitory factor (MIF), is highly expressed in various cancers and contributes to tumor progression by regulating inflammatory responses and the tumor microenvironment. The present study aimed to explore the role of macrophage migration inhibitory factors in cervical squamous cell carcinoma (CSCC) recurrence and metastasis. Western blotting, reverse transcription quantitative PCR, cell-counting kit 8 assay, flow cytometry, and ELISA were used to investigate the effects of MIF on CSCC progression and formation of inflammasomes. Transcriptome and proteome sequencing were combined to screen for key effector proteins of MIF. Moreover, in vitro co-culture experiments, Transwell assays, and flow cytometry were used to evaluate the roles of MIF and TSC22 domain family protein 3 (TSC22D3) as inflammatory tumor-promoting factors in macrophage recruitment and polarization induction. The results indicated that MIF was highly expressed in CSCC with lymph node metastasis, positively correlated with cervical cancer stage, and associated with poor prognosis. MIF was found to promote CSCC progression and linked to inflammasome activation. Multi-omics screening results indicated that TSC22D3 may be a crucial interacting gene of MIC. Moreover, MIF and TSC22D3 could facilitate inflammasome activation, macrophage recruitment, and M2 polarization. Therefore, MIF and TSC22D3 can induce macrophage infiltration in cervical cancer lesions and affect the tumor microenvironment by polarizing macrophages toward the M2 phenotype, promoting CSCC progression. This study highlights the potential of targeting the MIF-TSC22D3 axis as a novel therapeutic strategy and offering a promising avenue for improving the efficacy of immunotherapy in treatin

Dataset Information

DIA quantitative protein mass spectrometry of RBE cells knockout NAT10

Dataset's files

Publications

NAT10/ac<sup>4</sup>C drives intrahepatic cholangiocarcinoma by suppressing transposable elements via chromatin remodeling.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

DIA quantitative protein mass spectrometry of RBE cells knockout NAT10

Dataset's files

Publications

NAT10/ac&lt;sup&gt;4&lt;/sup&gt;C drives intrahepatic cholangiocarcinoma by suppressing transposable elements via chromatin remodeling.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

NAT10/ac<sup>4</sup>C drives intrahepatic cholangiocarcinoma by suppressing transposable elements via chromatin remodeling.