Dataset Information

Early innate immune response and evolution of a SARS-CoV-2 furin cleavage site inactive variant in bat cells

ABSTRACT: SARS-CoV-2 has caused the largest known coronavirus pandemic and is believed to have emerged from insectivorous bats. Little is known about the evolution of these viruses in their reservoir bat species. In this study, we investigated SARS-CoV-2-host interaction using human and bat cells. Bat cells mount a robust and early antiviral response but elicit a dampened pro-inflammatory response upon SARS-CoV-2 infection compared to human cells.

INSTRUMENT(S): maXis

ORGANISM(S): Homo Sapiens (human) Severe Acute Respiratory Syndrome Coronavirus 2 Eptesicus Fuscus

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Covid-19

SUBMITTER: Yoan Machado

LAB HEAD: Arinjay Banerjee

PROVIDER: PXD058807 | Pride | 2025-06-05

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	Calu3_24h_1_C2_1_273.d.zip	Other
	Calu3_24h_2_C5_1_285.d.zip	Other
	Calu3_24h_3_C8_1_297.d.zip	Other
	Calu3_24h_4_C11_1_309.d.zip	Other
	Calu3_24h_Mock1_C1_1_270.d.zip	Other

Items per page:

1 - 5 of 26

Similar Datasets

Project description:Bats are the most important natural reservoirs for a variety of emerging viruses that cause several illnesses in humans and other mammals. Increased viral shedding by bats is thought to be linked to an increased ability of many bat species to tolerate viral infection. The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19, is thought to have originated in bats, since viruses with high sequence similarity have been detected in bat feces. However, there is no robust in vitro model for assessing the SARS-CoV-2 infection in the bat GI tract. Here, we established gastrointestinal organoid cultures from Jamaican fruit bats (JFB, Artibeus jamaicensis), which replicated the characteristic morphology of the gastrointestinal epithelium and showed tissue specific gene expression patterns and cell differentiation. To analyze whether JFB intestinal epithelial cells are susceptible to SARS-CoV-2, we performed in vitro infection experiments. Increased SARS-CoV-2 RNA was found in both cell lysates and supernatants from the infected organoids after 48 h, and sgRNA also was detected, indicating that the JFB intestinal epithelium supports limited viral replication. However, no infectious virus was released into the culture media, and no cytopathic effects were observed. Gene expression studies revealed a significant induction of type I interferon and inflammatory cytokine genes in response to active SARS-CoV-2 virus but not to TLR agonist treatment. Untargeted analysis of the organoid proteome using data-independent acquisition mass spectrometry (DIA-MS) revealed a significant increase in proteins and pathways associated with inflammatory signaling, cell turnover and repair, and SARS-CoV-2 infection. Collectively, our data suggest that primary intestinal epithelial cells from JFBs are largely resistant to SARS-CoV-2 infection and cell damage, likely because they are able to mount a strong antiviral interferon and regenerative response upon infection.

Dataset Information

Early innate immune response and evolution of a SARS-CoV-2 furin cleavage site inactive variant in bat cells

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets