Proteomics

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SAPrIm 2.0: A semi-automated protocol for mid-throughput soluble HLA immunopeptidomics

ABSTRACT: Human leukocyte antigen (HLA) molecules are pivotal in guiding human adaptive immune responses through their presentation of peptide ligands, collectively known as the immunopeptidome. This process is central to the development of cancer immunotherapies, such as vaccines and T-cell therapies. Profiling the immunopeptidome from plasma and other biofluids has gained increasing traction, as it offers a minimally invasive approach for monitoring disease states and immune responses toward cancer therapy. Here we present the second iteration of SAPrIm, a refined immunopeptidomics tool optimized for soluble HLA analysis. It can process up to 12 samples per batch within a day. This workflow is positioned to advance the field of immunopeptidomics by enabling efficient plasma-based comparative analyses and mid-size cohort studies.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma

SUBMITTER: ERWIN TANUWIDJAYA  

LAB HEAD: Pouya Faridi

PROVIDER: PXD058880 | Pride | 2025-05-26

REPOSITORIES: Pride

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SAPrIm 2.0: a semi-automated protocol for mid-throughput soluble HLA immunopeptidomics.

Tanuwidjaya Erwin E   Lim Kam Sian Terry C C TCC   Steele Joel R JR   Goncalves Gabriel G   Woodhouse Isaac B IB   Chang Janet J   Ooi Joshua D JD   Schittenhelm Ralf B RB   Faridi Pouya P  

Frontiers in immunology 20250424

Human leukocyte antigen (HLA) molecules are pivotal in guiding human adaptive immune responses through their presentation of peptide ligands, collectively known as the immunopeptidome. This process is central to the development of cancer immunotherapies, such as vaccines and T-cell therapies. Profiling the immunopeptidome from plasma and other biofluids has gained increasing traction, as it offers a minimally invasive approach for monitoring disease states and immune responses toward cancer ther  ...[more]

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