COMPARATIVE PROTEOME ANALYSIS OF BIKANERI AND JAISALMERI CAMEL MILK IN INDIA

ABSTRACT: Camel milk is considered one of the most valuable food sources for nomadic people in arid and semi-arid areas and has also been consumed as a natural adjuvant for managing a variety of human diseases for centuries due to its nutritional values and extraordinary medicinal properties. A total of 180 milk samples were collected from Bikaneri and Jaisalmeri camels during various seasons in the Jodhpur and Sikar districts of Rajasthan for the proteomics study. Protein profiling of camel milk was done by LC-MS/MS. The protein pellets were prepared from 50 mL of pooled raw milk. The trypsinization and clean-up protocol was performed using standard methods. Then, Mass spectrometric analysis of peptide mixtures was performed using the EASY-nLC 1200 system coupled to a Thermo Fisher-Q Exactive equipped with a nanoelectrospray ion source. All samples were processed and analyzed with Proteome Discoverer (v2.4) against the Uniprot camel reference proteome database. A total of 704 protein groups were identified in the milk of Bikaneri and Jaisalmeri camels on the Uniprot Camelus dromedarius Database. There were 21 (3%) and 17 (2.4%) unique protein groups with 666 (94.6%) common proteins in the milk of Bikaneri and Jaisalmeri, respectively. In addition, 687 (97%) and 683 (98%) specific proteins were found in Bikaneri and Jaisalmeri camel milk, respectively. The camel casein components (CNs) were found as αs1-CN, αs2-CN, β- CN and κ-CN. Whey protein such as β-lactoglobulin was not found in the camel milk but α- lactalbumin was abundant. Breed variation was less observed because only 18 significantly expressed proteins were obtained in the milk of the Bikaneri and Jaisalmeri camel breeds.

INSTRUMENT(S):

ORGANISM(S): Camelus Dromedarius (dromedary) (arabian Camel)

SUBMITTER: Barkha Gupta  

LAB HEAD: Dr. Barkha

PROVIDER: PXD058940 | Pride | 2025-09-22

REPOSITORIES: Pride