Dataset Information

Study on the changes of extracellular matrix morphology and components in COPD animal model by using lung decellularized scaffold

ABSTRACT: Airway remodeling is a critical pathological process that influences the progression of chronic obstructive pulmonary disease（COPD）. To better study small airway remodeling in COPD, we employed advanced techniques such as decellularized scaffolds and proteomics to analyze compositional changes in the extracellular matrix（ECM）. Proteomic analysisidentified 70 differentially expressed proteins between the COPD group and the control group. These included 34 upregulated proteins such as Smarca2, Skt, Acvrl1, Myl2 (all with ratios > 10.64), and 36 downregulated proteins such as Col6a6, Col6a5, AnK3 (all with ratios < 0.27). Pathway analysis indicated activation of apoptosis (Enrichment Score, ES=0.23) and epithelial-mesenchymal transition (ES=0.38) genes, as well as inhibition of collagen synthesis (ES=-0.43) and degradation (ES=-0.63) genes were observed in the COPD group. These findings enhance our understanding of the mechanisms underlying airway remodeling and provide a scientific basis for developing novel therapeutic strategies for COPD.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Lung

DISEASE(S): Chronic Obstructive Pulmonary Disease

SUBMITTER: Lihua Xie

LAB HEAD: Lihua Xie

PROVIDER: PXD058944 | Pride | 2025-05-07

REPOSITORIES: Pride

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Study on the changes of extracellular matrix morphology and components in COPD animal model by using lung decellularized scaffold.

Li Yuan Y Dai Yingbing Y Jin Ting T Liu Xianyang X Xie Lihua L

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 20250301 6

Airway remodeling is a critical pathological process that influences the progression of chronic obstructive pulmonary disease(COPD). To better study small airway remodeling in COPD, we employed advanced techniques such as decellularized scaffolds, immunofluorescence, scanning electron microscopy, and proteomics to analyze morphological and compositional changes in the extracellular matrix (ECM). Our study revealed significant ultrastructural abnormalities in the decellularized scaffolds from the ...[more]

PMID: 40150895

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