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Extracellular vesicles of murine-derived epicardial derived cell line under hypoxic and normoxic environment TMT LC-MS

ABSTRACT: Extracellular vesicles (EVs) are key players in intercellular communication. Here we show that modulating cellular environment by changing the available oxygen levels in vitro in embryonic-derived epicardial-derived cells (EPDCs) from mice we modulate their protein content. Murine EPDCs react by secreting EV with increasing content in glycolysis-related proteins when in hypoxic environment.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Jorge Cobos

LAB HEAD: Jorge Cobos Figueroa

PROVIDER: PXD059112 | Pride | 2025-12-01

REPOSITORIES: Pride

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			Action	DRS
	210924-TMT10plex_ZT_TR1.raw	Raw
	210924-TMT10plex_ZT_TR2.raw	Raw
	210924-TMT10plex_ZT_TR3.raw	Raw
	210924-TMT10plex_x3TR.mzTab	Mztab

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Publications

Revealing the complexity of the epicardial secretome.

Oliveira Cláudia C CC Córdoba José J Pearson John R JR Guruceaga Elizabeth E Marín-Sedeño Ernesto E López-Moreno María M Guadix Juan Antonio JA García-Caballero Melissa M Pérez-Pomares José M JM Ruiz-Villalba Adrián A

Scientific reports 20251121 1

The epicardium, an epithelial layer covering the heart, plays pivotal roles in embryonic heart development and responses to adult cardiac damage. Epicardial-secreted molecular agents are known to be involved in the regulation of these phenomena, but how this regulation occurs is poorly understood. In this study, we have investigated extracellular vesicle (EV) and extracellular matrix (ECM) components of the epicardial secretome using a continuous mouse embryonic epicardial-derived cell (EPIC) li ...[more]

PMID: 41271808

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