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Nucleolar beacon for monitoring nucleolar morphology and proteomics in living cells

ABSTRACT: A novel approach combined nucleolar beacon with proximity labeling to obtain the nucleolar proteome. These techniques offer effective tools for investigating nucleolar functions in living cells and present a potential application method for drug delivery.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: jiajia gao

LAB HEAD: Jia Jia Gao

PROVIDER: PXD059125 | Pride | 2025-06-23

REPOSITORIES: Pride

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Publications

Nucleolar beacon for monitoring nucleolar morphology and proteomics in living cells.

Gao Jiajia J Sun Wenjie W Chen Yuting Y Liu Tianqi T Ge Zhenhuang Z Xu Wenwen W Lu Yongjun Y Wang Weiqiang W Ma Jinming J Ge Honghua H

Science advances 20250611 24

The nucleolus is essential for ribosome biogenesis and stress regulation. However, because of its dynamic nature, there is still a lack of methods to specifically visualize nucleolar localization in living cells and to study dynamic changes in protein interaction networks within the cell nucleolus. In this study, we identified and engineered a signal peptide sequence, termed nucleolar beacon, which exhibits robust nucleolar localization and universal applicability across various mammalian cell t ...[more]

PMID: 40498842

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