Dataset Information

Shotgun proteomics analysis by LC-ESI-MS/MS in pregnant women with excessive gingival bleeding

ABSTRACT: Pregnancy induces significant physiological, immunological, and hormonal changes, particularly elevated levels of estrogen and progesterone, which increase susceptibility to periodontal inflammation. Gingivitis, the early stage of periodontal inflammation, is primarily triggered by dental biofilm accumulation. This study aimed to investigate the pathophysiological mechanisms of gingivitis during pregnancy by analyzing the salivary proteomic profile of pregnant women with and without excessive gingival bleeding. Pregnant women were categorized into two groups: those with excessive gingival bleeding on probing (BOP > 50%; G1, n = 9) and those without generalized gingivitis (BOP < 30%; G2, n = 9). A comprehensive full-mouth periodontal examination was performed. Unstimulated whole-mouth saliva samples were collected and individually processed using Nano Liquid Chromatography Electron Spray Ionization Tandem Mass Spectrometry (nLC- ESI-MS/MS). Proteins were considered significantly downregulated at p < 0.05 and upregulated at 1-p > 0.95. Proteomic analysis identified 187 salivary proteins, with 75 shared between groups. Among these, 60 proteins were upregulated and 7 were downregulated in G1, with key upregulated proteins including Protein S100-A9 (16-fold increase), Neutrophil Defensins 1 and 3 (7-fold increase), Protein S100-A8 (5-fold increase), Beta-2-Microglobulin (4-fold increase), and several immunoglobulin isoforms, while Histatin-3 was notably the only protein downregulated by more than 2-fold. Gene Ontology analysis revealed significant enrichment in processes related to antimicrobial humoral response, bacterial defense mechanisms, and immune regulation, reflecting the inflammatory state. These findings provide novel insights into the salivary proteomic alterations associated with gingivitis in pregnancy, emphasizing the heightened antimicrobial and immune activity linked to excessive gingival inflammation.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Saliva

SUBMITTER: Gerson Foratori Junior

LAB HEAD: Gerson Aparecido

PROVIDER: PXD059154 | Pride | 2026-02-17

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	PRIDE_PaperClovis_IA.zip	Other
	PRIDE_PaperClovis_RAW.zip	Other

Items per page:

1 - 2 of 2

Similar Datasets

Project description:Objectives: Arterial hypertension (AH) influences salivary gland physiology and oral health, being associated with a higher incidence of periodontal disease in pregnant women. Evidence points to a bidirectional relationship between the oral microbiota and blood pressure regulation. Therefore, this study aimed to characterize the oral health of pregnant women and AH-associated changes in the salivary proteome and microbiome during pregnancy and postpartum. Design: Ten healthy women and ten women with AH were enrolled. Saliva was collected during pregnancy and six months postpartum. The salivary proteome was characterized by shotgun label-free mass spectrometry analysis. Specific proteins were validated through parallel reaction monitoring (PRM). The oral microbiota was characterized via 16S rRNA gene amplicon sequencing (V4 region). The periodontal health and the caries history was assessed during pregnancy. Results: Pregnant women with AH had lower junction plakoglobin (JUP)- and desmoplakin (DSP)-specific peptide levels than healthy women, confirmed by the PRM approach. The levels of these proteins correlated negatively with periodontal health indexes, which were higher in pregnant women with AH. In AH, nitrate-reducing microorganisms had lower abundance, correlating positively with JUP and DSP-specific peptides. Conclusions: The salivary proteome and microbiota are shaped by AH during and after pregnancy. Further research is required to understand the underlying mechanisms impairing oral health in AH. Data acquisition has been supported by EPIC-XS, project number 393, funded by the Horizon 2020 program of the European Union and the National Institute for Neurological Research (Programme EXCELES, ID Project No. LX22NPO5107) and the MEYS/EU project OP JAK-MULTIOMICS_CZ - Multi-omics platform for the search for biological correlates of diseases and the development of new diagnostic, preventive and therapeutic procedures (CZ.02.01.01/00/23_020/0008540).

Project description:HIV/SIV-associated periodontal disease (gingivitis/periodontitis) (PD) represents a major comorbidity affecting HIV patients on anti-retroviral therapy. Employing a systems biology approach, we report molecular changes underlying PD and its modulation by phytocannabinoids [delta-9-tetrahydrocannabinol (THC)] in uninfected and SIV-infected rhesus macaques (RMs) treated with vehicle (VEH/SIV) or THC (THC/SIV). VEH/SIV but not THC/SIV RMs showed significant enrichment of genes linked to anti-viral defense, interferon-beta, NFkB, RIG-1, and JAK-STAT signaling. We focused on the anti-microbial DUOX1 and immune activation marker IDO1 that were reciprocally regulated in gingiva of VEH/SIV RMs. Both proteins localized to the gingival epithelium and CD163+ macrophages, and showed differential expression in the gingiva of THC/SIV and VEH/SIV RMs. Additionally, inflammation-associated miR-21, miR-142-3p, miR-223, and miR-125a-5p showed significantly higher expression in the gingiva of VEH/SIV RMs. In human primary gingival epithelial cells, miR-125a-5p post-transcriptionally downregulated DUOX1 and THC inhibited IDO1 protein expression through a cannabinoid receptor-2 mediated mechanism. Interestingly, THC/SIV RMs showed relatively reduced plasma levels of kynurenine, kynurenate, and the neurotoxic quinolinate compared to VEH/SIV RMs. Most importantly, THC blocked HIV/SIV-induced depletion of Firmicutes and Bacteroidetes, and reduced Gammaproteobacteria abundance in saliva. Reduced IDO1 protein expression was associated with significantly (p<0.05) higher abundance of Prevotella, Lactobacillus (L. salivarius, L. buchneri, L. fermentum, L. paracasei, L. rhamnosus, L. johnsonii) and Bifidobacteria and reduced abundance of the pathogenic Porphyromonas cangingivalis and Porphyromonas macacae. These translational findings suggest that phytocannabinoids could help reduce gingival/systemic inflammation and salivary dysbiosis in ART naïve and treated HIV individuals.

Dataset Information

Shotgun proteomics analysis by LC-ESI-MS/MS in pregnant women with excessive gingival bleeding

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets