Proteomics

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A Bacteroides fragilis protease activates host PAR2 to induce intestinal pain and inflammation

ABSTRACT: Protease-activated receptor 2 (PAR2) is a central regulator of intestinal barrier function, inflammation and pain. Upregulated intestinal proteolysis and PAR2-signaling are implicated in inflammatory bowel diseases (IBDs) and irritable bowel syndrome (IBS), conditions often associated with gut microbiome alterations. To identify potential bacterial regulators of PAR2 activity, we developed a functional assay for PAR2 processing and screened a library of diverse gut microbes. We found that multiple bacteria secrete proteases that cleave host PAR2. Using chemoproteomic profiling with a covalent irreversible inhibitor, we uncovered a previously uncharacterized Bacteroides fragilis serine protease 1 (Bfp1) and show that it cleaves and activates PAR2 in multicellular and murine models. PAR2 cleavage by Bfp1 disrupts the intestinal barrier, sensitizes nociceptors, and triggers colonic inflammation and abdominal pain. Collectively, our findings uncover Bfp1-mediated PAR2-processing as a new axis of host-commensal-interaction in the gut that has the potential to be targeted for therapeutic intervention in IBD or IBS.

INSTRUMENT(S):

ORGANISM(S): Bacteroides Fragilis (strain Atcc 25285 / Nctc 9343)

SUBMITTER: Markus Lakemeyer  

LAB HEAD: Matthew Bogyo

PROVIDER: PXD059166 | Pride | 2025-09-28

REPOSITORIES: Pride

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Bacteroidesfragilis_NCTC9343_chromosome-plasmid-isoforms.fasta Fasta
Bogyo_11635_1_MLB174_B.fragilis.raw Raw
checksum.txt Txt
mqpar.xml Xml
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Publications

Protease-activated receptor 2 (PAR<sub>2</sub>) is a central regulator of intestinal barrier function, inflammation, and pain. Upregulated intestinal proteolysis and PAR<sub>2</sub> signaling are implicated in inflammatory bowel diseases (IBDs) and irritable bowel syndrome (IBS), conditions often associated with gut microbiome alterations. To identify potential bacterial regulators of PAR<sub>2</sub> activity, we developed a functional assay for PAR<sub>2</sub> processing to screen a library of  ...[more]

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