Quantitative proteomics of cytokine-induced memory-like (CIML) NK cells

ABSTRACT: NK cells from healthy donors were isolated from peripheral blood and stimulated overnight with IL-12/15/18 to induce a memory-like phenotype. After 7 days of culture, the phenotype and degranulation potential of CIML NK cells was characterized in two distinct NK cell subsets: CD16+/CD56+ and CD16−/CD56. Finally, NK cell subsets were purified using fluorescence-activated cell sorting (FACS) and subjected to high-throughput multiplexed quantitative proteomics.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Natural Killer Cell Activation Involved In Immune Response

SUBMITTER: Inmaculada Jorge  

LAB HEAD: Jesús María Vázquez Cobos

PROVIDER: PXD059190 | Pride | 2025-07-14

REPOSITORIES: Pride