Dataset Information

Illumina NovaSeq 6000 Mannose-B from Codonopsis Pilosula Modulates LAMB1 Expression to Enhance Trophoblast Function and Alleviate Subchorionic Hematoma

ABSTRACT: Introduction: This study investigates the active component Mannose-B from Codonopsis pilosula and its effect on human trophoblast cell function, particularly focusing on the regulation of Laminin Subunit Beta 1 (LAMB1) expression and its implications in subchorionic hematoma (SCH). Methods: Key genes involved in SCH pathology were identified through RNA sequencing and bioinformatics analysis. Network pharmacology was utilized to screen active components in Codonopsis pilosula and their critical targets. In vitro, HTR-8/Svneo cells were used to assess proliferation, migration, and invasion through CCK8, Transwell, and cell migration assays. A SCH rat model was established to evaluate changes in coagulation parameters, litter size, fetal viability, and fetal and placental weights. In vivo validation of Mannose-B's effects on LAMB1 expression and SCH pathology was performed using RT-qPCR and Western Blot. Results: Network pharmacology and molecular docking identified Mannose-B as an effective compound in Codonopsis pilosula, potentially beneficial for SCH treatment, with LAMB1 as a significant target. In vitro experiments showed that Mannose-B enhanced HTR-8/Svneo cell proliferation, migration, and invasion by reducing LAMB1 expression. In vivo experiments confirmed Mannose-B's inhibitory effect on placental LAMB1 expression and its potential in ameliorating SCH pathology. Conclusion: Mannose-B from Codonopsis pilosula inhibits LAMB1 expression, promoting human placental trophoblast cell proliferation, migration, and invasion, thereby mitigating the progression of SCH pathology.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Embryo

SUBMITTER: Qian Sun

LAB HEAD: Sun Qian

PROVIDER: PXD059213 | Pride | 2025-10-20

REPOSITORIES: Pride

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Mannose-B from Codonopsis pilosula modulates LAMB1 expression to enhance trophoblast function and alleviate subchorionic hematoma.

Sun Qian Q Gao Yuan Y Asan Kadirya K Ji Jing J Xu Jinjin J Wu Xiaoyan X Ren Jie J Feng Wen W Song Mengwei M Wang Sen S Zhang Boyu B Zhou Ying Y Han Conghui C Liu Shun S Yang Wen W

Scientific reports 20250811 1

This study investigates the active component Mannose-B from Codonopsis pilosula and its effect on human trophoblast cell function, particularly focusing on the regulation of Laminin Subunit Beta 1 (LAMB1) expression and its implications in subchorionic hematoma (SCH). Key genes involved in SCH pathology were identified through RNA sequencing and bioinformatics analysis. Network pharmacology was utilized to screen active components in Codonopsis pilosula and their critical targets. In vitro, HTR- ...[more]

PMID: 40789880

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