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Effects of MINCH exposure on the phosphoproteome of differentiating human SGBS adipocytes

ABSTRACT: Here, we evaluated the molecular effects of MINCH exposure, as primary metabolite of the DEHP substitute DINCH, on the phosphoproteome profiles of differentiating SGBS adipocytes. This study includes data on rosiglitazone differentiated controls, 10 µM MINCH exposure, and exposure to the selective PPAR inhibitor GW9662, as well as combinations thereof.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Stromal Cell

DISEASE(S): Simpson-golabi-behmel Syndrome Type 1

SUBMITTER: Alix Sarah Aldehoff

LAB HEAD: Kristin Schubert

PROVIDER: PXD059416 | Pride | 2025-08-05

REPOSITORIES: Pride

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			Action	DRS
	145_SGBS_GWMINCH_6_1.raw	Raw
	145_SGBS_GWMINCH_6_2.raw	Raw
	145_SGBS_GWMINCH_6_3.raw	Raw
	145_SGBS_GWMINCH_6_4.raw	Raw
	145_SGBS_GW_4_1.raw	Raw

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Publications

Advanced Proteomics Approaches Hold Potential for the Risk Assessment of Metabolism-Disrupting Chemicals as Omics-Based NAM: A Case Study Using the Phthalate Substitute DINCH.

Aldehoff Alix Sarah AS Karkossa Isabel I Broghammer Helen H Krupka Sontje S Weiner Juliane J Goerdeler Cornelius C Nuwayhid Rima R Langer Stefan S Wabitsch Martin M Rolle-Kampczyk Ulrike U Klöting Nora N Blüher Matthias M Heiker John T JT von Bergen Martin M Schubert Kristin K

Environmental science & technology 20250730 31

The concept of metabolic disruption through exposure to chemicals has expanded our understanding of how environmental pollution can contribute to metabolic dysregulation and, ultimately, diseases like obesity. New strategies for assessing the risks posed by chemicals are needed, and omics technologies, including proteomics, have proven to be powerful tools for investigating the molecular mechanisms of these metabolism-disrupting chemicals (MDCs). A potential MDC is the plasticizer DINCH─an alter ...[more]

PMID: 40736767

Similar Datasets

Effects of MINCH exposure on the proteome of differentiating human SGBS adipocytes

Project description:Here, we evaluated the molecular effects of MINCH exposure, as primary metabolite of the DEHP substitute DINCH, on the proteome of differentiating SGBS adipocytes. This study includes data on rosiglitazone differentiated controls, 10 µM MINCH exposure, and exposure to the selective PPAR inhibitor GW9662, as well as combinations thereof.

2025-08-05 | PXD059428 | Pride

Effects of MINCH exposure on the acetylome of differentiating human SGBS adipocytes

Project description:Here, we evaluated the molecular effects of MINCH exposure, as primary metabolite of the DEHP substitute DINCH, on the acetylome profiles of differentiating SGBS adipocytes. This study includes data on rosiglitazone differentiated controls, 10 µM MINCH exposure, and exposure to the selective PPAR inhibitor GW9662, as well as combinations thereof.

2025-08-05 | PXD059390 | Pride

Identification of potential protein interaction candidates of MINCH in SGBS adipocytes using Thermal Proteome Profiling (TPP)

Project description:Here, we identified potential protein interaction partners of MINCH, as primary metabolite of the phthalate DEHP substitute DINCH, in the human SGBS adipocyte cell line. This study includes data on the thermal stability of proteins upon incubation of the cell lysates with 10 µM MINCH at day 4, an early, and day 12, the terminal day of adipocyte differentiation.

2025-08-05 | PXD059386 | Pride

Effects of DINCH and MINCH exposure on the proteome of differentiating primary human stromal vascular fraction cells

Project description:Here, we evaluated the molecular effects of 10 nM and 10 µM DINCH and MINCH exposure on the proteome profiles of differentiating human primary SVF cells. The SVF cells derived from the subcutaneous adipose tissue of donors undergoing aesthetic surgery at abdomen, leg or back. The dataset comprised male and female donors.

2025-08-05 | PXD059419 | Pride

Effects of dietary DINCH exposure on the proteome, phosphoproteome and acetylome of adipose tissue of a murine diet induced model of obesity

Project description:Here, we evaluated the molecular effects of dietary DINCH exposure, on the proteome, phosphoproteome and acetylome profiles of visceral and subcutaneous adipose tissue in a model of diet-induced obesity in male and female C57BL/6N mice. This study includes data on visceral and subcutaneous adipose tissue of male and female mice that were either fed a standard high-fat diet (HFD) or two HFD diets including doses of DINCH (4,500 ppm and 15,000 ppm).

2025-08-05 | PXD059421 | Pride

Acute TNF-induced repression of cell identity genes is mediated by NFkB-directed redistribution of cofactors from super-enhancers

Project description:The proinflammatory cytokine tumor necrosis factor (TNF) plays a central role in low-grade adipose tissue inflammation and development of insulin resistance during obesity. In this context, nuclear factor kappa-light-chain-enhancer of activated B cells (NFkB), is directly involved and required for the acute activation of the inflammatory gene program. Here we show that the major transactivating subunit of NF?B, v-rel avian reticuloendotheliosis viral oncogene homolog A (RELA), is also required for acute TNF-induced suppression of adipocyte genes. Notably, this repression does not involve RELA binding to the associated enhancers but rather loss of cofactors and enhancer RNA (eRNA) selectively from high occupancy sites within super-enhancers. Based on these data we have developed models that with high accuracy predict which enhancers and genes are repressed by TNF in adipocytes. We show that these models are applicable to other cell types where TNF represses genes associated with super-enhancers in a highly cell type-specific manner. Our results propose a novel paradigm for NF?B-mediated repression, whereby NF?B selectively redistributes cofactors from high occupancy enhancers, thereby specifically repressing super-enhancer-associated cell identity genes. Genome-wide assesment of the acute transcriptional response to TNF in human SGBS adipocytes using RNA- ChIP- and DHS-seq. Total RNA-seq and RNAPII-ChIP seq for vehicle and TNF treated adipocytes are available under GSE60462

2015-06-17 | E-GEOD-64233 | biostudies-arrayexpress

Phosphoproteome dynamics during differentiation of SGBS adipocytes

Project description:Here, we identified temporal dynamics of the phosphoproteome during adipocyte differentiation in the human SGBS cells, applying untargeted proteomics in combination with enrichment strategies for phosphorylated peptides. Samples were taken at initiation of differentiation, at an intermediate and terminal time point.

2024-06-13 | PXD045770 | Pride

Phosphatases modified by LH signaling in ovarian follicles: testing their role in regulating the NPR2 guanylyl cyclase

Project description:In response to luteinizing hormone (LH), multiple proteins in rat and mouse granulosa cells are rapidly dephosphorylated, but the responsible phosphatases remain to be identified. Because the phosphorylation state of phosphatases can regulate their interaction with substrates, we searched for phosphatases that might function in LH signaling by using quantitative mass spectrometry. We identified all proteins in rat ovarian follicles whose phosphorylation state changed detectably in response to a 30-min exposure to LH, and within this list, identified protein phosphatases or phosphatase regulatory subunits that showed changes in phosphorylation. Phosphatases in the phosphoprotein phosphatase (PPP) family were of particular interest because of their requirement for dephosphorylating the natriuretic peptide receptor 2 (NPR2) guanylyl cyclase in the granulosa cells, which triggers oocyte meiotic resumption. Among the PPP family regulatory subunits, PPP1R12A and PPP2R5D showed the largest increases in phosphorylation, with 4–10 fold increases in signal intensity on several sites. Although follicles from mice in which these phosphorylations were prevented by serine-to-alanine mutations in either Ppp1r12a or Ppp2r5d showed normal LH-induced NPR2 dephosphorylation, these regulatory subunits and others could act redundantly to dephosphorylate NPR2. Our identification of phosphatases and other proteins whose phosphorylation state is rapidly modified by LH provides clues about multiple signaling pathways in ovarian follicles.

2023-11-20 | PXD033878 | Pride

Transcriptome responses in copepods Calanus hyperboreus exposed to phenanthrene and benzo[a]pyrene

Project description:The arctic ecosystems are increasingly exposed to pollution particularly from offshore petroleum extraction-related activities. The objective of the study is to map transcriptome responses in copepods of the arctic region in response to PAHs phenanthrene (Phe) and benzo[a]pyrene (BaP) found in crude-oil contaminants.

2021-10-01 | E-MTAB-10918 | biostudies-arrayexpress

Transcriptome responses in copepods Calanus finmarchicus exposed to phenanthrene and benzo[a]pyrene

2021-10-01 | E-MTAB-10917 | biostudies-arrayexpress